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Merck
CN

A3660

Sigma-Aldrich

Apoferritin from equine spleen

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About This Item

CAS Number:
9013-31-4
MDL number:
MFCD00081365
UNSPSC Code:
12352202
NACRES:
NA.25

Key Documents

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form

glycerol solution (50% glycerol and 0.075 M NaCl)

Quality Level

200

packaging

vial of 50 mg

storage temp.

−20°C

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Application

Apoferritin is a gel filtration molecular weight marker that can be used in gel filtration chromatography and protein chromatography. Apoferritin has the ability to demetallate hemin and to metallate protoporphyrin IX.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

低风险生物材料

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000362475
0000362474
0000362475
0000362474
0000262612

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  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. What is the molecular weight of Product A3660, Apoferritin from equine spleen?

    The approximate molecular weight of this product is 443,000.

  4. What is the concentration of Product A3660, Apoferritin from equine spleen?

    The concentration is roughly 25 mg/mL, according to the technical bulletin.

  5. Can Product A3660, Apoferritin from equine spleen, be sterilized?

    Yes. First the glycerol will need to be removed (typically by dialysis). Then a solution of apoferritin in 0.15M sodium chloride can be filtered through a 0.2 micron filter, as is done for Product No. A3641.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

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    Ask a Scientist here.

David P Klebl et al.
Acta crystallographica. Section D, Structural biology, 76(Pt 4), 340-349 (2020-04-08)
Despite the great strides made in the field of single-particle cryogenic electron microscopy (cryo-EM) in microscope design, direct electron detectors and new processing suites, the area of sample preparation is still far from ideal. Traditionally, sample preparation involves blotting, which
Noëlle Carette et al.
Journal of inorganic biochemistry, 100(8), 1426-1435 (2006-06-20)
Earlier crystallographic and spectroscopic studies had shown that horse spleen apoferritin was capable of removing the metal ion from hemin (Fe(III)-protoporphyrin IX) [G. Précigoux, J. Yariv, B. Gallois, A. Dautant, C. Courseille, B. Langlois d'Estaintot, Acta Cryst. D50 (1994) 739-743;
Venkata P Dandey et al.
Nature methods, 17(9), 897-900 (2020-08-12)
We present an approach for preparing cryo-electron microscopy (cryo-EM) grids to study short-lived molecular states. Using piezoelectric dispensing, two independent streams of ~50-pl droplets of sample are deposited within 10 ms of each other onto the surface of a nanowire EM
Richard Henderson et al.
Microscopy (Oxford, England), 62(1), 43-50 (2013-01-08)
Theoretical considerations together with simulations of single-particle electron cryomicroscopy images of biological assemblies in ice demonstrate that atomic structures should be obtainable from images of a few thousand asymmetric units, provided the molecular weight of the whole assembly being studied
Patrycja Kaczara et al.
Pigment cell & melanoma research, 25(6), 804-814 (2012-08-14)
Melanosomes were recently shown to protect ARPE-19 cells, a human retinal pigment epithelium (RPE) cell line, against oxidative stress induced by hydrogen peroxide. One postulated mechanism of antioxidant action of melanin is its ability to bind metal ions. The aim
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