A3981
Anti-Beta (β)-Amyloid antibody
mouse monoclonal, BAM-10
Synonym(s):
Anti-A-BETA, Anti-Amyloid β Precursor Protein, Clone BAM91
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About This Item
Conjugate:
unconjugated
Clone:
BAM-10, monoclonal
Application:
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
indirect ELISA
western blot
indirect ELISA
western blot
Species reactivity:
human
Citations:
19
Technique(s):
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5-5 μg/mL using formic-acid treated tissue sections of human Alzheimer disease brain tissue
indirect ELISA: suitable
western blot: suitable
indirect ELISA: suitable
western blot: suitable
Uniprot accession no.:
Product Name
Anti-β-Amyloid antibody, Mouse monoclonal, clone BAM-10, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
BAM-10, monoclonal
form
buffered aqueous solution
species reactivity
human
packaging
antibody small pack of 25 μL
enhanced validation
independent
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concentration
~1.5 mg/mL
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 2.5-5 μg/mL using formic-acid treated tissue sections of human Alzheimer disease brain tissue
indirect ELISA: suitable
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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Application
Monoclonal Anti-β-Amyloid antibody has been used in immunoprecipitation analysis, microarray-based immunoassay and microarray.
Mouse monoclonal anti-ABETA was used to treat old WT PDAPP mice with amyloid accumulation and learning deficits in an attempt to improve learning and decrease accumulation, however no response was observed.
The antibody is useful in immunohistochemistry, immunoblotting, ELISA, and competitive ELISA. Also, this antibody has been used to neutralize Aβ assemblies in brains of transgenic mice expressing a mutant form of amyloid precursor protein, and for in vivo deep tissue imaging using near-IR optical spectrum.
Biochem/physiol Actions
β-amyloid gene acts as the substrate of insulin-degrading enzyme (IDE). It plays a major role in the pathogenesis of Alzheimer′s disease (AD) and type 2 diabetes mellitus (DM2).
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
β-amyloid gene is located on human chromosome 21q21.
The β-amyloid precursor protein (APP) is cleaved sequentially by the proteolytic enzymes β-and γ-secretase to produce β-amyloid (Aβ) peptides with the Aβ1-42 and the Aβ1-40 forms being the most prevalent. Secreted Aβ peptides are degraded either via a re-uptake mechanism followed by endosomal degradation, or by an extracellular insulin degrading enzyme. Extracellular accumulation of Aβ leads to the formation of aggregates, fibrils and eventually amyloid deposits called neuritic plaques, which is the hallmark of Alzheimer′s disease (AD).
The antibody reacts specifically with β-amyloid protein. The epitope recognized by the antibody resides within amino acids 1-12 of the β-amyloid protein. It specifically stains amyloid plaques within the cortex and amyloid deposits in blood vessels using formic acid-treated, formalin-fixed, paraffin-embedded, and Methacarn-fixed sections of human Alzheimer′s disease (AD) brain tissue.
Immunogen
Synthetic β-amyloid peptide, conjugated to KLH.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
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Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Regulatory Information
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Antibodies targeted to the brain with image-guided focused ultrasound reduces amyloid-β plaque load in the TgCRND8 mouse model of Alzheimer's disease.
Jord?o JF, et al.
PLoS ONE, 5(5), e10549-e10549 (2010)
Interactions of pathological hallmark proteins: Tubulin polymerization promoting protein/p25, β-amyloid and α-synuclein.
Olah J, et al.
The Journal of Biological Chemistry, jbc-M111 (2011)
Mercedes M Pérez-Jiménez et al.
Nature communications, 12(1), 49-49 (2021-01-06)
Aging and fertility are two interconnected processes. From invertebrates to mammals, absence of the germline increases longevity. Here we show that loss of function of sul-2, the Caenorhabditis elegans steroid sulfatase (STS), raises the pool of sulfated steroid hormones, increases
Application of immunosignatures to the assessment of Alzheimer's disease.
Restrepo L, et al.
Annals of Neurology, 70(2), 286-295 (2011)
Alexandra Petiet et al.
Methods in molecular biology (Clifton, N.J.), 771, 293-308 (2011-08-30)
Alzheimer's disease (AD) is an important social and economic issue for our societies. The development of therapeutics against this severe dementia requires assessing the effects of new drugs in animal models thanks to dedicated biomarkers. According to the amyloid cascade
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