biological source
rabbit
Quality Level
conjugate
peroxidase conjugate
antibody form
affinity isolated antibody
antibody product type
secondary antibodies
clone
polyclonal
form
buffered aqueous solution
species reactivity
goat
should not react with
human
technique(s)
direct ELISA: 1:10,000
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
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General description
IgG antibodies regulate several functions such as complement activation and phagocytosis. Thus they play a crucial role in facilitating cytological immune responses. Anti-goat IgG (whole molecule)–peroxidase antibody can be used as a secondary antibody in ovarian tissue microarray. Rabbit anti-goat IgG (whole molecule)-peroxidase antibody reacts specifically with all goat IgG but shows no reactivity with human serum proteins.
Immunogen
Purified goat IgG.
Application
Anti-Goat IgG (whole molecule)-Peroxidase antibody produced in rabbit is suitable for use in immunoblot and immunohistochemistry. The product can also be used for direct ELISA (1:10,000).
The presence of pleiotrophin in HUVEC cell culture medium was analyzed by western blot using HRP-conjugated rabbit anti-goat IgGas the secondary at a dilution of 1:7500 in TBST.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.
Preparation Note
Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).
Other Notes
Antibody adsorbed with human serum proteins.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Regulatory Information
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