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A4721

Sigma-Aldrich

Monoclonal Anti-ARNO (Cytohesin-2) antibody produced in mouse

~2 mg/mL, clone CYT2-21, purified immunoglobulin, buffered aqueous solution

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About This Item

MDL number:
MFCD03454755
UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

CYT2-21, monoclonal

form

buffered aqueous solution

mol wt

antigen 40 kDa

species reactivity

rat, human

concentration

~2 mg/mL

technique(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 5-10 μg/mL using a whole extract of rat brain

isotype

IgG1

UniProt accession no.

Q99418

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CYTH2(9266)
rat ... Cyth2(116692)

General description

ARNO (Cytohesin-2) is a guanine-nucleotide exchange factor that belongs to Arf family of GTP-associated proteins. ARNO has been shown to modulate the movement of mouse preadipocyte 3T3-L1 cells. Studies have reported that cytohesin-2 is overexpressed in pulmonary cancers and can also serve as biomarker of prognosis in hepatocellular carcinomas.
Monoclonal Anti-ARNO (Cytohesin-2) reacts specifically with ARNO, and does not cross-react with other members of the cytohesin family. The antibody associates with human and rat ARNO.

Immunogen

recombinant, human ARNO (ARFnucleotide-binding-site opener).

Application

Mouse monoclonal anti-ARNO (Cytohesin-2) antibody can be used for western blot (5-10μg/mL using a whole extract of rat brain), microarray, indirect ELISA and immunocytochemistry applications.
To determine if ARNO binds to phosphoinositides, a protein-overlay assay was performed using monoclonal mouse anti-ARNO as the primary antibody for ARNO detection on membranes spotted with phosphoinositides and incubated with ARNO.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Lot/Batch Number
070M4764
091K4869

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