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A6007

Apotryptophanase from Escherichia coli

soluble powder, 75-150 units/mg solid

Synonym(s):

Tryptophanase from Escherichia coli, L-Tryptophan indole-lyase (deaminating)

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About This Item

CAS Number:
9024-00-4
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
4.1.99.1 (BRENDA, IUBMB)
MDL number:
MFCD00130539
Specific activity:
75-150 units/mg solid
Biological source:
Escherichia coli
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biological source

Escherichia coli

Quality Segment

form

soluble powder

specific activity

75-150 units/mg solid

storage temp.

−20°C

Application

Apotryptophanase is used for the quantitative determination of pyridoxal-phosphate. Apotryptophanase, from Sigma, has been used to study pregnancy-associated PLP deficiency and vitamin B-6 deficiency .

Biochem/physiol Actions

Apotryptophanase hydrolizes tryptophan and is capable of catalyzing α,β-elimination reactions with a number of substituted amino acids, including S-methyl-, S-ethyl- and S-benzyl- L-cysteine. DTNB inactivates tryptophanase .

Other Notes

One unit releases one μg of indole from L-tryptophan in 10 min at pH 8.3 at 37 °C in the presence of 0.04 mM pyridoxal-5′--phosphate.

Storage Class

11 - Combustible Solids

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

PPE (personal protective equipment)

Eyeshields, Gloves, type N95 (US)

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