A6007
Apotryptophanase from Escherichia coli
soluble powder, 75-150 units/mg solid
Synonym(s):
Tryptophanase from Escherichia coli,
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
75-150 units/mg solid
Biological source:
Escherichia coli
biological source
Escherichia coli
Quality Segment
form
soluble powder
specific activity
75-150 units/mg solid
storage temp.
−20°C
Application
Apotryptophanase is used for the quantitative determination of pyridoxal-phosphate. Apotryptophanase, from Sigma, has been used to study pregnancy-associated PLP deficiency and vitamin B-6 deficiency .
Biochem/physiol Actions
Apotryptophanase hydrolizes tryptophan and is capable of catalyzing α,β-elimination reactions with a number of substituted amino acids, including S-methyl-, S-ethyl- and S-benzyl- L-cysteine. DTNB inactivates tryptophanase .
Other Notes
One unit releases one μg of indole from L -tryptophan in 10 min at pH 8.3 at 37 °C in the presence of 0.04 mM pyridoxal-5′--phosphate.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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