A6805
β-N-Acetylglucosaminidase from Streptococcus pneumoniae
recombinant, expressed in E. coli, buffered aqueous solution
Synonym(s):
β-N-Acetyl-D-hexosaminide N-acetylhexosaminohydrolase, β-N-Acetylhexosaminidase
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.32
EC Number:
232-730-2
MDL number:
Specific activity:
≥80 units/mg protein
Recombinant:
expressed in E. coli
recombinant
expressed in E. coli
Quality Segment
form
buffered aqueous solution
specific activity
≥80 units/mg protein
packaging
vial of ≥1.0 unit
foreign activity
β-galactosidase, α-mannosidase, α-fucosidase, neuraminidase, and proteases, none detected (Enzyme is expressed in glycosidase-free host.)
shipped in
wet ice
storage temp.
2-8°C
Gene Information
Streptococcus pneumoniae R6 ... lytB(934406)
Application
β-N-Acetylglucosaminidase from Streptococcus pneumoniae has been used in the removal of O-linked sugars from inositol 1,4,5-trisphosphate (InsP3) receptor type I. It has also been used in coating PVDF strips for amelogenin trityrosyl motif peptide (ATMP) and keratin 5 binding studies.
Biochem/physiol Actions
β-N-Acetylglucosaminidase from Streptococcus pneumoniae is an extracellular glycosidase and has broad specificity over the β1-3 and β1-6 linkages present in mucins. It belongs to the glycoside hydrolase family 85 (GH85). Also called endo-β-N-acetylglucosaminidase, it cleaves at the asparagine residue of the di-N-acetylchitobiose structure in oligosaccharides and acts on fucosylated N-glycan core. Its deglycosylation functionality is exploited in studying functions of glycoproteins.
Specific for GlcNAc; not significantly active with GalNAc
This enzyme, sometimes called β-N-acetylhexosaminidase, is reported to liberate terminal β-linked N-acetylglucosamine and N-acetylgalactosamine from a variety of substrates.
Physical form
Solution in 20 mM Tris-HCl, pH 7.5, 25 mM NaCl
Other Notes
One unit will hydrolyze 1.0 μmole of p-nitrophenyl N-acetyl-β-D-glucosaminide to p-nitrophenol and N-acetyl-D-glucosamine per min at the pH 5 at 37 °C.
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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