A7347
2-Aminoacridone
≥98% (non-aqueous titration), solid
Synonym(s):
2-Amino-9(10H)-acridinone, 2-amino-10H-acridin-9-one, AMAC
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About This Item
Empirical Formula (Hill Notation):
C13H10N2O
CAS Number:
Molecular Weight:
210.23
UNSPSC Code:
12352200
Beilstein/REAXYS Number:
172520
MDL number:
InChI
1S/C13H10N2O/c14-8-5-6-12-10(7-8)13(16)9-3-1-2-4-11(9)15-12/h1-7H,14H2,(H,15,16)
InChI key
PIGCSKVALLVWKU-UHFFFAOYSA-N
assay
≥98% (non-aqueous titration)
form
solid
solubility
DMF: soluble, DMSO: soluble
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Application
2-Aminoacridone is a highly fluorescent aromatic, which contains a primary amine group that reacts with an aldehyde group at the reducing end of a carbohydrate and is reduced to a stable amine derivative by sodium borohydride (NaBH4). Picomolar levels of glycan compounds can be detected using this fluorophore. The resulting derivatzed compounds can be separated by reverse-phase HPLC and detected by positive-ion electrospray MS . An intense fluorescent, hydrophobic probe that is stable over a wide pH range is useful in the derivatization of glycans to allow for the analysis of complex oligosaccharides using micellar electrokinetic capillary chromatography and reverse- and normal-phase chromatography coupled with mass spectroscopy to determine relative concentrations and structural identity of individual oligosaccharides . The λ excitation and λ emission are 425 nm and 532 nm, respectively.
Fluorescent label for glycans and saccharides. The analysis of fluorophore-labeled glycans by high-resolution polyacrylamide gel electrophoresis.
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