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A7481

Sigma-Aldrich

Anti-Atg1/ULK1 antibody produced in rabbit

enhanced validation

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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Synonym(s):
Anti-UNC51, Anti-Unc-51 like kinase 1 (C. elegans), Anti-Unc51.1
NACRES:
NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~150 kDa

species reactivity

rat, human (predicted), mouse

enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

concentration

~1 mg/mL

technique(s)

western blot: 0.5-1.0 μg/mL using whole extracts of HEK-293T cells expressing mouse ULK1
western blot: 3-6 μg/mL using whole extracts of rat PC12

UniProt accession no.

O75385

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ULK1(8408)
mouse ... Ulk1(22241)
rat ... Ulk1(360827)

Related Categories

General description

Atg1 is an autophagy protein that functions as a serine/threonine kinase in yeast to regulate autophagasome formation. Atg1 is ULK1 in mammals.
Unc-51 like autophagy activating kinase 1 (ULK1) gene is located on human chromosome 12q24.33.

Specificity

Anti- Atg1/ULK1 recognizes rat and mouse Atg1/ULK1.

Immunogen

Synthetic peptide corresponding to amino acids of mouse Atg1/ULK1 conjugated to KLH via a cysteine residue. The corresponding sequence is identical in rat and differs by 3 amino acids in human.

Application

Anti-Atg1/ULK1 antibody produced in rabbit has been used in western blotting and immunohistochemical staining.

Biochem/physiol Actions

Unc-51 like autophagy activating kinase 1 (ULK1) regulates autophagy initiation and innate immunity of interferons (IFNs). ULK1 is critical for neuronal development. It is essential for neurite elongation and differentiation of cerebellar granule neurons. Mutations in this gene is linked to latent mycobacterium tuberculosis infection (LTBI).

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Gene responsible for mitochondrial myopathy and sideroblastic anemia (MSA) maps to chromosome 12q24. 33
Casas K, et al.
American Journal of Medical Genetics. Part A, 127(1), 44-49 (2004)
Noboru Mizushima et al.
Nature cell biology, 12(9), 823-830 (2010-09-03)
It has been known for many decades that autophagy, a conserved lysosomal degradation pathway, is highly active during differentiation and development. However, until the discovery of the autophagy-related (ATG) genes in the 1990s, the functional significance of this activity was
Beyond autophagy: New roles for ULK1 in immune signaling and interferon responses
Saleiro D, et al.
Cytokine & Growth Factor Reviews, 29, 17-22 (2016)
Taichi Hara et al.
The Journal of cell biology, 181(3), 497-510 (2008-04-30)
Autophagy is a membrane-mediated intracellular degradation system. The serine/threonine kinase Atg1 plays an essential role in autophagosome formation. However, the role of the mammalian Atg1 homologues UNC-51-like kinase (ULK) 1 and 2 are not yet well understood. We found that
Human ULK1 variation and susceptibility to Mycobacterium tuberculosis infection
Horne DJ, et al.
The Journal of Infectious Diseases, 214(8), 1260-1267 (2016)
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