A7718
ArrayHyb™ Hybridization Buffer
Sign Into View Organizational & Contract Pricing
Select a Size
About This Item
MDL number:
UNSPSC Code:
12161700
General description
In response to the need for a hybridization buffer with high signal-to-noise ratios in fluorescent microarray analysis, Sigma has developed ArrayHyb™ and ArrayHyb™ LowTemp Microarray Hybridization Buffers.
ArrayHyb and ArrayHyb LowTemp are optimized, low-viscosity, ready-to-use hybridization buffers designed for use in gene expression analysis with cDNA or oligonucleotide probes spotted onto glass slides. Hybridizations with fluorescently labeled nucleic acids consistently provide superior signal and minimal background compared to standard and other commercially available hybridization buffers. Both formulations increase the rate of hybridization and can provide signals for analytical purposes in as little as 2 hours. Approximately 60-80% of the maximum signal is obtained in six hours, with extended incubation times resulting in increased sensitivity without increased background.
ArrayHyb is designed to replace standard microarray hybridization buffers when hybridizing at temperatures greater than 60°C.
This flexibility of ArrayHyb buffers allows hybridizations to be tailored to meet your specific requirements. ArrayHyb buffers are not only flexible, but also versatile and may be used with PCR products, genomic DNA, or oligonucleotides probes arrayed on glass slides.
ArrayHyb and ArrayHyb LowTemp are optimized, low-viscosity, ready-to-use hybridization buffers designed for use in gene expression analysis with cDNA or oligonucleotide probes spotted onto glass slides. Hybridizations with fluorescently labeled nucleic acids consistently provide superior signal and minimal background compared to standard and other commercially available hybridization buffers. Both formulations increase the rate of hybridization and can provide signals for analytical purposes in as little as 2 hours. Approximately 60-80% of the maximum signal is obtained in six hours, with extended incubation times resulting in increased sensitivity without increased background.
ArrayHyb is designed to replace standard microarray hybridization buffers when hybridizing at temperatures greater than 60°C.
This flexibility of ArrayHyb buffers allows hybridizations to be tailored to meet your specific requirements. ArrayHyb buffers are not only flexible, but also versatile and may be used with PCR products, genomic DNA, or oligonucleotides probes arrayed on glass slides.
Sigma offers optimized, low-viscosity, ready-to-use hybridization buffers designed for use in gene expression analysis with probes spotted onto glass slides. ArrayHyb buffers may be used with PCR products, genomic DNA, or oligonucleotides probes arrayed on glass slides.
Application
ArrayHyb is an optimized microarray hybridization buffer recommended for use when hybridizing at temperatures greater than 60 °C.
Features and Benefits
- Increased rate of hybridization
- Superior signal and minimal background compared to standard and other commercially available hybridization buffers
- Signals for analytical purposes provided in as little as 2 hours.
- 60-80% of the maximum signal is obtained in six hours; extended incubation times result in increased sensitivity without increased background
Legal Information
ArrayHyb is a trademark of Sigma-Aldrich Co. LLC
Regulatory Information
新产品
This item has
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service