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Merck
CN

A8103

Sigma-Aldrich

Anti-hABH1 antibody, Mouse monoclonal

clone hABH1-151, purified from hybidoma cell culture

Synonym(s):

Anti-Human AlkB Homologue 1, Monoclonal Anti-hABH1 antibody produced in mouse

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About This Item

MDL number:
MFCD06412250
UNSPSC Code:
12352203
NACRES:
NA.41

Key Documents

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biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

hABH1-151, monoclonal

form

buffered aqueous solution

mol wt

antigen ~45 kDa

species reactivity

human, monkey

technique(s)

immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using total cell extract of 293T cells

isotype

IgG1

UniProt accession no.

Q13686

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... ALKBH1(8846)

General description

Anti-hABH1 antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the hABH1-151 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to a fragment of human hABH1, conjugated to keyhole limpet hemocyanin (KLH).
Human AlkB homologue 1 (hABH1) is one of the eight homologs of E. coli AlkB. Homology modeling studies have analyzed the role of hABH1 gene in DNA repair after alkaylation-induced damage.

Application

Anti-hABH1 antibody, Mouse monoclonal has been used in
  • chromatin immunoprecipitation(ChIP)
  • immunohistochemistry
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunoprecipitation

Biochem/physiol Actions

Monoclonal Anti-hABH1 (human AlkB homologue) recognizes human and monkey ABH1. It does not recognize hABH2 or hABH3. The antibody epitope resides within amino acids 19-34 of human hABH1.
The human homolog, AlkB homolog 1, histone H2A dioxygenase (Alkbh1) is linked with several enzymatic actions, such as, DNA, mRNA or tRNA hydroxylation, cleavage at abasic (AP) site in DNA and demethylation of histones. It plays a key role in epigenetic gene silencing, as it is known to exhibit m6A demethylation activity . ALKBH1 participates in normal cell proliferation.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
126M4807V
034K4812

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Characterization of human AlkB homolog 1 produced in mammalian cells and demonstration of mitochondrial dysfunction in ALKBH1-deficient cells
Muller TA, et al.
Biochemical and biophysical research communications, 495(1), 98-103 (2018)
Role of ALKBH1 in the core transcriptional network of embryonic stem cells
Ougland R, et al.
Cellular Physiology and Biochemistry, 38(1), 173-184 (2016)
Shankaracharya et al.
Interdisciplinary sciences, computational life sciences, 3(3), 175-181 (2011-10-01)
Inhibition of DNA repair mechanism through alkylating agents in tumor cells is an important method for cancer treatment. Alkylation damage repair gene AlkB was first reported in E. coli. In human and other mammals eight distinguishing homologs of AlkB were
Rune Ougland et al.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 38(1), 173-184 (2016-01-15)
ALKBH1, an AlkB homologue in the 2-oxoglutarate and Fe2+ dependent hydroxylase family, is a histone dioxygenase that removes methyl groups from histone H2A. Studies of transgenic mice lacking Alkbh1 reveal that most Alkbh1-/- embryos die during embryonic development. Embryonic stem
Rune Ougland et al.
Stem cells (Dayton, Ohio), 30(12), 2672-2682 (2012-09-11)
AlkB homolog 1 (ALKBH1) is one of nine members of the family of mammalian AlkB homologs. Most Alkbh1(-/-) mice die during embryonic development, and survivors are characterized by defects in tissues originating from the ectodermal lineage. In this study, we

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