A9048
4-Azidobenzoic acid N-hydroxysuccinimide ester
≥95%, powder
Synonym(s):
N-Hydroxysuccinimidyl 4-azidobenzoate
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About This Item
Empirical Formula (Hill Notation):
C11H8N4O4
CAS Number:
Molecular Weight:
260.21
UNSPSC Code:
12352200
MDL number:
SMILES string
N=[N]=Nc1ccc(cc1)C(=O)ON2C(=O)CCC2=O
assay
≥95%
form
powder
solubility
chloroform: 50 mg/mL, DMF: soluble
storage temp.
−20°C
Application
Photoactive, heterobifunctional cross-linking reagant. Typically, initial reaction couples via ester to primary amine by amide bond formation in the pH range 6.5-8.5. Second bonding occurs during UV irradiation (250 nm) via reactive nitrene. The latter bonding is rapid and non-specific.
Other Notes
Note that reducing agents such as thiols may reduce the azide to amine and should be avoided. Initial manipulations and coupling should be performed under reduced light.
Regulatory Information
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Photoaffinity labeling of peptide hormone binding sites.
R E Galardy et al.
The Journal of biological chemistry, 249(11), 3510-3518 (1974-06-10)
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