A9207
Avidin−Agarose from egg white
aqueous glycerol suspension
Synonym(s):
Avidin agarose
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About This Item
Form:
aqueous glycerol suspension
Biological source:
egg white
biological source
egg white
Quality Level
conjugate
agarose conjugate
form
aqueous glycerol suspension
matrix
cross-linked 6% beaded agarose
matrix activation
epichlorohydrin
matrix attachment
amino
matrix spacer
15 atoms
storage temp.
−20°C
General description
Avidin comprises of 0.05% of total egg white protein. It is a glycosylated protein. It corresponds to a molecular weight of 16.5 kDa and exists as tetramer, which can bind a minimum of three biotin molecules.
Application
Avidin-Agarose from egg white has been used:
- in the precipitation of biotin tagged protein by pull down assay in neuroblastoma cell line (SH-SY5Y )
- in the purification of Lewis(x) sugar binding proteins from Schistosoma mansoni
- in avidin bead assay for purification of labelled biotin
- for capture of glycoproteins from murine cornea samples
Avidin is used for the purification of biotin containing proteins, which can enhance cellular binding and internalization of the antibody (Ab)-functionalized capsules compared with control human immunoglobulin (IgG)-functionalized capsules, suggesting these capsules can specifically interact with cancer cells through antibody/antigen recognition.
Biochem/physiol Actions
Avidin finds biotechnological applications due to its high-affinity ligand binding property. Avidin interaction with biotin through its carbohydrate chains is widely exploited in biotechnological applications. In cancer cells, avidin-biotin systems helps in the in vivo localization and drug targeting in tumor cells.
Physical form
Suspension in 50% glycerol, 0.01 M sodium phosphate, pH 7.0, containing 0.02% sodium azide.
Other Notes
One unit will bind 1.0 μg of d-biotin.
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
低风险生物材料
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Efficient production of active chicken avidin using a bacterial signal peptide in Escherichia coli
Hytonen VP, et al.
The Biochemical Journal, 384(2), 385-390 (2004)
Yumi Abiko et al.
Free radical biology & medicine, 104, 118-128 (2017-01-04)
The current consensus is that environmental electrophiles activate redox signal transduction pathways through covalent modification of sensor proteins with reactive thiol groups at low concentrations, while they cause cell damage at higher concentrations. We previously exposed human carcinoma A431 cells
Systematic evaluation of avidin-biotin interaction by fluorescence spectrophotometry
Sun, S., et al.
Spectrochimica Acta Part A: Molecular Spectroscopy, 89C, 99-104 (2011)
Covalent modification of cytoskeletal proteins in neuronal cells by tryptamine-4, 5-dione
Kato Y, et al.
Redox Biology, 2, 983-990 (2014)
Jens Spross et al.
Analytical and bioanalytical chemistry, 402(7), 2395-2405 (2012-01-21)
A poly(glycidyl methacrylate-co-acrylamide-co-ethylene dimethacrylate) monolith and a poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith were prepared in fused silica capillaries (100 μm ID) and modified with monomeric avidin using the glutaraldehyde technique. The biotin binding capacity of monolithic affinity columns with immobilized monomeric
Related Content
利用亲和力、GST pull-down、TAP 和共免疫沉淀方法，通过Pull-Down研究体外蛋白质互作。
Investigate in vitro protein-protein interactions with pull-down assays, utilizing affinity, GST pull-down, TAP, and co-immunoprecipitation methods.
Global Trade Item Number
| SKU | GTIN |
|---|---|
| A9207-1ML | 04061822683532 |
| A9207-5ML | 04061822683549 |
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