A9329
Aldolase from spinach
lyophilized powder, 0.3-1.0 units/mg protein
Synonym(s):
D-Fructose-1,6-bisphosphate-D-glyceraldehyde-3-phosphate-lyase
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About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
232-781-0
MDL number:
form
lyophilized powder
specific activity
0.3-1.0 units/mg protein
shipped in
dry ice
storage temp.
−20°C
General description
Aldolase exists in three isoforms namely: ALDOA, ALDOB, and ALDOC. These isoenzymes are differentially expressed in human tissues.
Application
Aldolase from spinach has been used as a standard in electrophoresis for the detection of mesocarp and seed aldolase proteins.
Biochem/physiol Actions
Aldolase catalyzes the conversion of fructose 1,6-bisphosphate (F1,6BP) to glyceraldehyde-3-phosphate (G3P) and dihydroxyacetone phosphate (DHAP). It plays a vital role in metabolism and glycolysis. Other than glycolytic enzymes, aldolase also interacts with F-actin, α-tubulin, dynein light chain 8, nexin 9, and phospholipase D2, which indicates its role as a scaffolder and modulator of protein complexes. In addition, it also regulates cell morphology and acidic vesicle trafficking by directly interacting with the ADP-ribosylation factor nucleotide site opener (ARNO). Aldolase may also be involved in the formation of mannoheptulose and perseitol. Increased expression of the protein has been observed in various diseases and cancer types.
Analysis Note
Crude
Protein determined by Lowry
Other Notes
One unit will convert 1.0 μmole of fructose 1,6-diphosphate to dihydroxyacetone phosphate and glyceraldehyde 3-phosphate per min at pH 7.4 at 25 °C.
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Regulatory Information
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Yu-Chan Chang et al.
Trends in endocrinology and metabolism: TEM, 29(8), 549-559 (2018-06-17)
The aldolase family members involved in metabolism and glycolysis are present in three isoforms: ALDOA, ALDOB, and ALDOC. Aldolases are differentially expressed in human tissues, and aberrant expression has been observed in several human diseases and cancer types. However, non-enzymatic
D-mannoheptulose and perseitol in `Hass? avocado: Metabolism in seed and mesocarp tissue
Tesfay SZ , et al.
Southern Medical Journal, 79, 159-165 (2012)
Aldolase directly interacts with ARNO and modulates cell morphology and acidic vesicle distribution.
Maria Merkulova et al.
American journal of physiology. Cell physiology, 300(6), C1442-C1455 (2011-02-11)
Previously, we demonstrated that the vacuolar-type H(+)-ATPase (V-ATPase) a2-subunit functions as an endosomal pH sensor that interacts with the ADP-ribosylation factor (Arf) guanine nucleotide exchange factor, ARNO. In the present study, we showed that ARNO directly interacts not only with
Linjun Guo et al.
Acta crystallographica. Section F, Structural biology and crystallization communications, 68(Pt 8), 958-961 (2012-08-08)
4-Hydroxy-3-methyl-2-keto-pentanoate aldolase (asHPAL), an enzyme used in the synthesis of (2S,3R,4S)-4-hydroxyisoleucine, was crystallized in the absence and the presence of 2-ketobutyrate as one of its substrates by the sitting-drop vapour-diffusion method using PEG 400 as a precipitant. Crystals of asHPAL
Masahiko Hayakawa et al.
Chembiochem : a European journal of chemical biology, 13(15), 2191-2195 (2012-09-12)
Programming an anti-flu strategy: A new and potent neuraminidase inhibitor that maintains long-term systemic exposure of an antibody and the therapeutic activity of the neuraminadase inhibitor zanamivir has been created. This strategy could provide a promising new class of influenza
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