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Merck
CN

A9545

4-Aminophenyl β-D-galactopyranoside

≥98% (TLC), β-galactosidase substrate

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About This Item

Empirical Formula (Hill Notation):
C12H17NO6
CAS Number:
5094-33-7
Molecular Weight:
271.27
NACRES:
NA.25
PubChem Substance ID:
24891470
UNSPSC Code:
12352201
MDL number:
MFCD00067362

Key Documents

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InChI

1S/C12H17NO6/c13-6-1-3-7(4-2-6)18-12-11(17)10(16)9(15)8(5-14)19-12/h1-4,8-12,14-17H,5,13H2

SMILES string

Nc1ccc(OC2OC(CO)C(O)C(O)C2O)cc1

InChI key

MIAKOEWBCMPCQR-UHFFFAOYSA-N

assay

≥98% (TLC)

form

powder

optical activity

[α]/D -42.00 to -34.00°, c = 9.00-11.00 mg/mL in water

technique(s)

thin layer chromatography (TLC): suitable

color

white to yellow cast

solubility

water: 49.00-51.00 mg/mL, clear, colorless to yellow

storage temp.

−20°C

Quality Level

200

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Application

4-Aminophenyl β-D-galactopyranoside (PAPG, 4-APG) is used as a substrate for the development of β-galactosidase(s) (GUS)-dependent assay systems via the formation of an electroactive product. It may be used to create affinity matricies for the capture or purification of β-galactosidase(s).

Other Notes

To gain a comprehensive understanding of our extensive range of Monosaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

涉药品监管产品
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Certificates of Analysis (COA)

Lot/Batch Number
0000292633
0000258267
0000236980
0000258267
0000236980

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Olivier Laczka et al.
Analytica chimica acta, 641(1-2), 1-6 (2009-04-28)
Here a novel electrochemical method for the rapid detection of anti-HIV antibodies in serum is presented. The novelty lies in the combination of allosteric enzymes and coulometry to yield a fast, simple and reliable HIV diagnostic method. We have used
D P Sarkar et al.
Immunological communications, 11(3), 175-188 (1982-01-01)
Negatively-charged, multilamellar liposomes, covalently coupled with p-aminophenyl-beta-D-galactopyranoside, elicited the rabbits a nearly equivalent anti-galactosyl immune response in both the presence and the absence of complete Freund's-adjuvant (CFA). The antibody response obtained using liposomes both as the carrier and adjuvant was
Benoît Limoges et al.
Journal of the American Chemical Society, 128(18), 6014-6015 (2006-05-04)
Electrochemical responses (e.g., chronoamperometric) obtained with an immobilized enzyme that produces an electroactive species may be used to quantitate the amount of enzyme or the concentration of its substrate. It is shown, on theoretical and experimental bases, that product-to-substrate coupling
Takatoshi Kaya et al.
Chemical communications (Cambridge, England), (2)(2), 248-249 (2004-01-23)
[small beta]-Galactosidase expression in a small number of Escherichia coli cells has been measured in real time with an electrochemical sensor chip. E. coli cells were embedded using collagen gel within a micropore which was microfabricated onto a chip. The
[p-Aminophenyl-beta-D-galactopyranoside as an antigenic determinant in neoglycoproteins].
D M Belen'kiĭ
Molekuliarnaia biologiia, 17(1), 54-61 (1983-01-01)
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