A9655

Auramine O

Name: Auramine O
Brand: SIGMA

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Synonym(s):

4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2, Pyoctaninum aureum

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About This Item

Empirical Formula (Hill Notation):

C₁₇H₂₁N₃ · HCl

CAS Number:

2465-27-2

Molecular Weight:

303.83

UNSPSC Code:

12171500

NACRES:

NA.47

PubChem Substance ID:

24891484

EC Number:

219-567-2

MDL number:

MFCD00012484

Colour Index Number:

41000

Beilstein/REAXYS Number:

4030061

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Product Name

Auramine O, Dye content ≥80 %, certified by the BSC

InChI key

KSCQDDRPFHTIRL-UHFFFAOYSA-N

InChI

1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H

SMILES string

Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C

agency

certified by the BSC

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

color

yellow to green

mp

>250 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL, yellow to orange

λ_max

370 nm in H₂O
432 nm in H₂O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

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Related Categories

Clinical & Diagnostics

Dry Dyes & Certified Stains

Hematology Stains

Histology Stains

Tissue Diagnostics

Application

Auramine O has been used to study its absorption by a natural biopolymer poly (γ-glutamic acid).

General description

Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Auramine O is used for the staining of acid-fast organisms like coccidia. Along with carbol, it generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

pictograms

GHS08,GHS07,GHS09

signalword

Warning

hcodes

H302,H319,H351,H411

pcodes

P201 - P273 - P301 + P312 + P330 - P305 + P351 + P338 - P308 + P313

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

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E W Koneman.

Koneman's Color Atlas and Textbook of Diagnostic Microbiology, 1565-1565 (2006)

E Kohen.

Cell Structure and Function by Microspectrofluorometry, 490-490 (2014)

Equilibrium and kinetic studies on sorption of basic dyes by a natural biopolymer poly (γ-glutamic acid).

Inbaraj B S, et al.

Biochemical Engineering Journal, 31(3), 204-215 (2006)

Reactive dynamics in confined liquids: ultrafast torsional dynamics of auramine O in nanoconfined water in aerosol OT reverse micelles.

Ismael A Heisler et al.

The journal of physical chemistry. B, 113(6), 1623-1631 (2009-01-17)

The effects of confinement on the ultrafast torsional reaction of auramine O in aqueous solution are investigated through ultrafast fluorescence up-conversion with 50 fs time resolution. The aqueous solution is confined in nanoscale water droplets by an ionic surfactant. The

Fading of auramine-stained mycobacterial smears and implications for external quality assurance.

Jessica Minion et al.

Journal of clinical microbiology, 49(5), 2024-2026 (2011-03-25)

Light-emitting diode fluorescence microscopy is being scaled up for tuberculosis control, but fading of auramine-stained slides could compromise external quality assurance. We stored auramine-stained slides and reexamined them over time. Slides stored in all environments faded quickly, with significant changes

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Auramine O