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A9655

Auramine O

Dye content, ≥80%, certified by the Biological Stain Commission, powder

Synonym(s):

4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2, Pyoctaninum aureum

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About This Item

Empirical Formula (Hill Notation):
C17H21N3 · HCl
CAS Number:
2465-27-2
Molecular Weight:
303.83
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24891484
EC Number:
219-567-2
MDL number:
MFCD00012484
Colour Index Number:
41000
Beilstein/REAXYS Number:
4030061
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Product Name

Auramine O, Dye content ≥80 %, certified by the BSC

agency

certified by the BSC

Quality Level

200

form

powder

composition

Dye content, ≥80%

technique(s)

microbe id | staining: suitable

color

yellow to green

mp

>250 °C (dec.) (lit.)

solubility

ethanol: 1 mg/mL, yellow to orange

λmax

370 nm in H2O, 432 nm in H2O

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

SMILES string

Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C

InChI

1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H

InChI key

KSCQDDRPFHTIRL-UHFFFAOYSA-N

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General description

Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Auramine O is used for the staining of acid-fast organisms like coccidia. Along with carbol, it generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

Application

Auramine O has been used to study its absorption by a natural biopolymer poly (γ-glutamic acid).

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Warning

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
SHBT0566
SHBS7131
SHBR0463
SHBQ6204
SHBL5991

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E W Koneman.
Koneman's Color Atlas and Textbook of Diagnostic Microbiology, 1565-1565 (2006)
E Kohen.
Cell Structure and Function by Microspectrofluorometry, 490-490 (2014)
Equilibrium and kinetic studies on sorption of basic dyes by a natural biopolymer poly (γ-glutamic acid).
Inbaraj B S, et al.
Biochemical Engineering Journal, 31(3), 204-215 (2006)
M Habtamu et al.
The international journal of tuberculosis and lung disease : the official journal of the International Union against Tuberculosis and Lung Disease, 16(3), 402-407 (2012-05-30)
Mawenzi Regional Hospital, northern Tanzania. To determine the value of light-emitting diode (LED) microscopy in diagnosing tuberculosis (TB) on bleach-treated and direct sputum smears. Sputum samples were collected from patients suspected of pulmonary TB who presented consecutively at the laboratory
Jessica Minion et al.
Journal of clinical microbiology, 49(5), 2024-2026 (2011-03-25)
Light-emitting diode fluorescence microscopy is being scaled up for tuberculosis control, but fading of auramine-stained slides could compromise external quality assurance. We stored auramine-stained slides and reexamined them over time. Slides stored in all environments faded quickly, with significant changes
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Global Trade Item Number

SKUGTIN
A9655-25G04061835571017

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