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Merck
CN

A9811

Sigma-Aldrich

Alkaline Phosphatase-Anti-Alkaline Phosphatase antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

Synonym(s):

APAAP

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
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biological source

rabbit

antibody form

affinity isolated antibody

antibody product type

primary antibodies

form

buffered aqueous solution

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10

shipped in

wet ice

storage temp.

2-8°C

General description

Soluble complex of calf intestinal alkaline phophatase and rabbit antibodies to alkaline phosphatase.
The product may be used for immunohistology and immunocytology assays applying the Alkaline Phosphatase-Anti-Alkaline Phosphatase (APAAP) technique. This method involves the use of primary rabbit antibodies, a bridging antibody, the APAAP complex and a suitable substrate.

Application

Alkaline Phosphatase-Anti-Alkaline Phosphatase antibody has been used for IHC assays.
Immunohistochemistry was performed on parraffin embedded uteroplacental sections (5 um) using APAAP as the primary at a dilution of 1:100 in 0.5M TBS.

Physical form

Solution in 0.05 M Tris buffered saline, pH 8.0, containing 0.001 mM MgCl2, 1% bovine serum albumin, and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Transactivation Of Cellular Genes Involved In Nucleotide Metabolism By The Regulatory IE1 Protein Of Murine Cytomegalovirus Is Not Critical For Viral Replicative Fitness In Quiescent Cells And Host Tissues.
Wilhelmi, V., et al.
J. Virol. Methods, 82, 9900-9916 (2008)

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