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Merck
CN

A9986

Sigma-Aldrich

Albumin human

recombinant, expressed in rice, lyophilized powder, Immunoglobin-free, ≥96% (SDS-PAGE)

Synonym(s):

Albumin human, Albumin-DX, rHSA

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About This Item

Empirical Formula (Hill Notation):
C2936H4624N786O889S41
CAS Number:
Molecular Weight:
66471.53
EC Number:
MDL number:
UNSPSC Code:
12352202
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biological source

human

recombinant

expressed in rice

Assay

≥96% (SDS-PAGE)

form

lyophilized powder

mol wt

monomer ~67 kDa

technique(s)

cell culture | mammalian: suitable

NCBI accession no.

UniProt accession no.

InChI

1S/C3F8/c4-1(5,2(6,7)8)3(9,10)11

InChI key

QYSGYZVSCZSLHT-UHFFFAOYSA-N

Gene Information

human ... ALB(213)

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Application

This is a recombinant albumin that is animal component- and immunoglobin-free. This human albumin is suitable for use as a blocking agent for procedures such as ELISA, RIA, and western blotting and as a stabilizing agent for protein and analyte solutions. This albumin is not tested for suitablility for cell culture applications. For cell culture applications, please see the cell culture tested recombinant human albumins, Product No. A9731 and A6608.
This recombinant variant is recommended for use in absorption, distribution, metabolism, and excretion (ADME) pharmacological research; cell culture; drug delivery research; and cryopreservation of cells.

Biochem/physiol Actions

Albumins are soluble monomeric proteins found in the body fluids and tissues of animals and in some plant seeds. Serum albumin functions as a carrier protein for steroids, fatty acids, and thyroid hormones. Serum albumins are also vital in regulating the colloidal osmotic pressures of blood.

Preparation Note

Solutions of this albumin can be prepared by dissolving the powder in water and mixing or inverting gently (to prevent bubble formation). If a sterile solution is required, the solution can be filtered through a 0.2 μm filter. Solutions should be stored at 4-8° C. This albumin contains a small amount of NaCl to aid in solubility in water. A human albumin 20 % (w/v) solution will contain approximately 50 mM NaCl.

Other Notes

View more information on human serum albumin.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Information

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Marco Molari et al.
eLife, 9 (2020-06-17)
Affinity maturation is a complex dynamical process allowing the immune system to generate antibodies capable of recognizing antigens. We introduce a model for the evolution of the distribution of affinities across the antibody population in germinal centers. The model is
Klaus Eyer et al.
Journal of immunology (Baltimore, Md. : 1950), 205(4), 1176-1184 (2020-07-17)
One of the major goals of vaccination is to prepare the body to rapidly secrete specific Abs during an infection. Assessment of the vaccine quality is often difficult to perform, as simple measurements like Ab titer only partly correlate with
Jay Jagannathan et al.
Journal of neurosurgery, 109(3), 547-552 (2008-09-02)
Convection-enhanced delivery (CED) is increasingly used to investigate new treatments for central nervous system disorders. Although the properties of CED are well established in normal gray and white matter central nervous system structures, the effects on drug distribution imposed by
Mauricio Sendeski et al.
Radiology, 251(3), 697-704 (2009-04-16)
To determine whether a type of contrast medium (CM), iodixanol, modifies outer medullary descending vasa recta (DVR) vasoreactivity and nitric oxide (NO) production in isolated microperfused DVR. Animal handling conformed to the Animal Care Committee Guidelines of all participating institutions.
Abdullah Hussain et al.
Immunology, 128(2), 236-244 (2009-09-11)
Anti-proteinase 3 antibodies are implicated in the pathogenesis of small vessel vasculitis. These are primarily immunoglobulin G (IgG), with different subclasses predominating at different stages of disease. However, little is known of their respective roles in pathogenesis. We have previously

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