Anti-ACTB antibody produced in rabbit

Actin, a highly conserved protein, is a major component of both the cytoskeletal and contractile structures in all cell types. It varies in amount, being related to the type of differentiation and to the functional state of cells and tissues. Actin can be found in two different forms of aggregation, the globular or the fibrillar state, and at least six distinct isoforms occur in vertebrates. The actins exhibit over 90% sequence homology, but each isoform has a unique NH2-terminal sequence. The isoforms are comprised of three α actins (skeletal, cardiac, smooth), one β actin (β-non-muscle) and two gamma actins (gamma smooth muscle and gamma non-muscle). Difficulties have been encountered in producing polyclonal antibodies due to the highly conserved nature of actin.

synthetic peptide corresponding to a region of human ACTB with an internal ID of P22019

β-actin is a nonmuscle actin involved in cytoskeletal structures. Anti-ACTB (anti-β-Actin) is a rabbit IgG polyclonal antibody used to tag β-Actin protein(s) for detection and quantitation by Western blotting and in cells and tissues by immunohistochemical (IHC) techniques.
Rabbit Anti-ActinB is useful for studying actin structure and function, and to probe binding sites of actin-binding proteins.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Anti-ACTB (anti-β-Actin) antibody recognizes an internal sequence of human β-actin.

Lyophilized from PBS buffer with 2% sucrose

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