B0306
Anti-Bcl-10 (AQ-18) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
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About This Item
UNSPSC Code:
12352203
MDL number:
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~32 kDa
species reactivity
rat, human, mouse
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:250 using heat-retrieved, sections of rat spleen, immunoprecipitation (IP): 5-10 μg using lysate from 4-6x105 human THP-1/Raji cells, microarray: suitable, western blot: 1:2,500 using whole extract of human THP-1/Raji cells, western blot: 1:500 using whole extract of mouse NIH3T3 fibroblasts
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... BCL10(8915)
mouse ... Bcl10(12042)
rat ... Bcl10(83477)
General description
Bcl-10 or CIPER is an N-terminal CARD (Caspase Recruitment Domain) containing protein mapped on to chromosome 1 at 1p22 and is localized in perinuclear region of cytoplasm and in membrane raft along with DPP4.
Immunogen
synthetic peptide corresponding to the human Bcl-10 sequence (amino acids 216-233), with N-terminal lysine added, conjugated to KLH.
Application
Polyclonal anti-Bcl-10 (AQ-18) antibody produced in rabbit is suitable for immunoprecipitation at a concentration of 5-10μg by using lysate from 4-6x105 human THP-1/Raji cells and for immunohistochemistry at 1:250 working dilution using formalin-fixed, paraffin-embedded heat-retrieved, sections of rat spleen. The product is also suitable for western blot analysis at a working dilution of 1:2,500 using whole extract of human THP-1/Raji cells and at 1:500 using whole extract of mouse NIH3T3 fibroblasts. Further, anti-Bcl-10 (AQ-18) antibody has shown suitability for microarray analysis.
Biochem/physiol Actions
Bcl-10 is a cellular homolog of the equine herpesvirus-2 protein E-10 (vCLAP). It has been implicated in the regulation of apoptosis by interacting with caspase 9, enhancing procaspase 9 processing and triggering its activation when overexpressed in the cell. Deregulation of Bcl-10 expression leads to cellular oncogenesis.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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M Yan et al.
The Journal of biological chemistry, 274(15), 10287-10292 (1999-04-03)
Apoptotic signaling is mediated by homophilic interactions between conserved domains present in components of the death pathway. The death domain, death effector domain, and caspase recruitment domain (CARD) are examples of such interaction motifs. We have identified a novel mammalian
T Yoneda et al.
The Journal of biological chemistry, 275(15), 11114-11120 (2001-02-07)
To elucidate the function of Bcl10, recently cloned as an apoptosis-associated gene mutated in MALT lymphoma, we identified its binding partner TRAF2, which mediates signaling via tumor necrosis factor receptors. In mammalian cells, low levels of Bcl10 expression promoted the
T G Willis et al.
Cell, 96(1), 35-45 (1999-02-16)
MALT B cell lymphomas with t(1;14)(p22;q32) showed a recurrent breakpoint upstream of the promoter of a novel gene, Bcl10. Bcl10 is a cellular homolog of the equine herpesvirus-2 E10 gene: both contain an amino-terminal caspase recruitment domain (CARD) homologous to
Kei Ohnuma et al.
The Journal of biological chemistry, 282(13), 10117-10131 (2007-02-09)
CD26 is a widely distributed 110-kDa cell surface glycoprotein with an important role in T-cell costimulation. We demonstrated previously that CD26 binds to caveolin-1 in antigen-presenting cells, and following exogenous CD26 stimulation, Tollip and IRAK-1 disengage from caveolin-1 in antigen-presenting
Q Zhang et al.
Nature genetics, 22(1), 63-68 (1999-05-13)
Mucosa-associated lymphoid tissue (MALT) lymphomas most frequently involve the gastrointestinal tract and are the most common subset of extranodal non-Hodgkin lymphoma (NHL). Here we describe overexpression of BCL10, a novel apoptotic signalling gene that encodes an amino-terminal caspase recruitment domain
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