B0561
Monoclonal Anti-BUB1 antibody produced in mouse
2 mg/mL, clone 14H5, purified immunoglobulin, buffered aqueous solution
Synonym(s):
Anti-Budding Uninhibited By Benzimidazoles 1
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About This Item
Conjugate:
unconjugated
Clone:
14H5, monoclonal
Application:
ARR, ELISA (i), ICC, IP, WB
Citations:
6
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
14H5, monoclonal
form
buffered aqueous solution
species reactivity
human
concentration
2 mg/mL
technique(s)
immunocytochemistry: 4-8 μg/mL using HeLa cells treated with 20 μg/mL nacodazol, immunoprecipitation (IP): suitable, indirect ELISA: suitable, microarray: suitable, western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... BUB1(699)
General description
BUB1 is spindle checkpoint kinase that modulates kinetochore-independent segregation of chromosomes and cell proliferation. This kinase regulates the phosphorylation of MAD1 and itself. As a result, BUB1 mediates chromosome-spindle attachments during mitosis. BUB1 also phosphorylates Cdc20 which subsequently facilitates accurate checkpoint signaling . Monoclonal Anti-BUB1 antibody is specific for human BUB1 (approx. 150 kDa).
Immunogen
recombinant human BUB1.
Application
Monoclonal Anti-BUB1 antibody is suitable for use in western blot, indirect ELISA, microarray, immunocytochemistry (4-8 μg/mL using HeLa cells treated with 20 μg/mL nacodazol) and immunoprecipitation.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| B0561-200UL | 04061837909146 |