B21200
EnPresso® B Defined Nitrogen-free
Growth system for expressing protein in bacteria, Starter Pack
sterility
sterile; γ-irradiated
form
tablet
pH
6.8
shipped in
ambient
storage temp.
room temp
Biochem/physiol Actions
EnPresso® B Defined Nitrogen-free is a pre-sterilized, chemically-defined growth system designed to increase the yield of 15N-labelled proteins.
EnPresso® growth systems provide optimal conditions for growth, metabolism and protein expression in microbial cultures. Protein yields are increased by enabling cultures to reach far higher cell densities than those achieved using conventional media. By controlling growth rate and metabolism, a greater proportion of expressed protein can be correctly folded to improve solubility, minimize the risk of inclusion body formation, and ensure functionality of the final product.
EnPresso® growth systems maintain pH, provide adequate minerals, vitamins and trace elements to support growth, and use proprietary EnBase™ technology to ensure a constant, slow release of glucose from a "polysaccharide substrate.
See all available products from EnPresso B Growth Systems.
EnPresso® growth systems provide optimal conditions for growth, metabolism and protein expression in microbial cultures. Protein yields are increased by enabling cultures to reach far higher cell densities than those achieved using conventional media. By controlling growth rate and metabolism, a greater proportion of expressed protein can be correctly folded to improve solubility, minimize the risk of inclusion body formation, and ensure functionality of the final product.
EnPresso® growth systems maintain pH, provide adequate minerals, vitamins and trace elements to support growth, and use proprietary EnBase™ technology to ensure a constant, slow release of glucose from a "polysaccharide substrate.
See all available products from EnPresso B Growth Systems.
Physical form
EnPresso® B is supplied in a kit providing sufficient reagents for 4 separate 50 ml cultures. Included in the kit:
8 tablets in 4 blue bags
1 bottle (1 ml) Reagent A
8 tablets in 4 blue bags
1 bottle (1 ml) Reagent A
Other Notes
BioSilta performance guarantee for EnPresso Starter Packs:
For expression of recombinant proteins in E. coli, BioSilta guarantees a minimun 5-fold increase in protein yield from EnPresso B Defined nitrogen-free growth system when compared to yields from comercially available minimal media. Comparisons must be made using an EnPresso Starter Pack and a commercially-available growth media. Manufacturer′s protocols must be followed exactly. Cultures must be adequately aerated in shake flasks or 24 deepwell plates. Failure to achieve minimum 5-fold increase in yield must be reported to BioSilta (through Sigma Technical Services) within 21 days of product delivery and include quantitative data from the EnPresso growth system and the comparative growth media. Subject to data review, BioSilta/Sigma will refund the purchase price of the EnPresso Starter Pack. Please note this guarantee does not apply to membrane proteins and proteins expressed into the periplasm as performance data is not yet available.
For expression of recombinant proteins in E. coli, BioSilta guarantees a minimun 5-fold increase in protein yield from EnPresso B Defined nitrogen-free growth system when compared to yields from comercially available minimal media. Comparisons must be made using an EnPresso Starter Pack and a commercially-available growth media. Manufacturer′s protocols must be followed exactly. Cultures must be adequately aerated in shake flasks or 24 deepwell plates. Failure to achieve minimum 5-fold increase in yield must be reported to BioSilta (through Sigma Technical Services) within 21 days of product delivery and include quantitative data from the EnPresso growth system and the comparative growth media. Subject to data review, BioSilta/Sigma will refund the purchase price of the EnPresso Starter Pack. Please note this guarantee does not apply to membrane proteins and proteins expressed into the periplasm as performance data is not yet available.
Legal Information
EnBase is a trademark of BioSilta Oy
EnPresso is a registered trademark of BioSilta Oy
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Danger
Hazard Classifications
Aquatic Chronic 2 - Carc. 1A - Carc. 1B Inhalation - Skin Sens. 1 - STOT RE 2
Storage Class
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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