B2685
transformation
for protein expression and DNA plasmid production
Synonym(s):
BL21 Competent Cells
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About This Item
MDL number:
UNSPSC Code:
12352200
grade
Molecular Biology
description
T1R
form
suspension
shipped in
dry ice
storage temp.
−70°C
General description
BL21-T1R are competent E. coli that are suitable for high level expression/production of heterologous proteins regulated by various expression vector systems. The cells have transformation efficiency of ≥3x106 cfu/μg when transformed with non-saturating amounts of pUC19 plasmid DNA.
Sigma′s BL21-T1R competent E. coli cells are grown and made chemically competent using an optimized procedure specific to the strain, followed by strain verification and efficiency testing. The cells are provided in frozen 50 μl aliquots for convenience. Each aliquot can be used for a single transformation.
Application
Suitable for expression and production of proteins directed by a range of expression systems with promoters such as lac, trc, tac, λPL and araD
Biochem/physiol Actions
BL21-T1R does not express ion proteases and outer membrane protease, ompT. This prevents the degradation of heterologous proteins expressed by various expression vector systems. This strain also contains tonA genotype that confers resistance to lytic bacteriophages such as T1 and T5.
Features and Benefits
- Ensures recovery of heterologous proteins
- Contains the genotype tonA that protects the clonal stocks from lytic bacteriophages
- Guaranteed high transformation efficiency
- Convenient 50 μL aliquots
Other Notes
- BL21-T1R chemically competent cells, 10 X 50 μL (B2810)
- pUC 19 control DNA (10 ng/μL), 10 μL (D2567)
Storage Class Code
10 - Combustible liquids
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Losee L Ling et al.
Nature, 517(7535), 455-459 (2015-01-07)
Antibiotic resistance is spreading faster than the introduction of new compounds into clinical practice, causing a public health crisis. Most antibiotics were produced by screening soil microorganisms, but this limited resource of cultivable bacteria was overmined by the 1960s. Synthetic
Adam Johnson et al.
The EMBO journal, 34(6), 811-827 (2015-01-15)
In mammalian cells, cargo-laden secretory vesicles leave the endoplasmic reticulum (ER) en route to ER-Golgi intermediate compartments (ERGIC) in a manner dependent on the COPII coat complex. We report here that COPII-coated transport carriers traverse a submicron, TFG (Trk-fused gene)-enriched
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