B3760
Blue dextran−Agarose
saline suspension
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About This Item
UNSPSC Code:
23151817
MDL number:
form
saline suspension
Quality Level
extent of labeling
1-5 mg per mL
matrix
Cross-linked 4% beaded agarose
matrix activation
cyanogen bromide
matrix attachment
amino
matrix spacer
1 atom
storage temp.
2-8°C
Application
Blue dextran is used in affinity chromatography as a protein binding site. It has been used to study Alzheimer′s disease by quantifying cerebrospinal fluid (CSF) in relation to amyloid accumulation in rat brain parenchyma.
Physical form
Suspension in 0.5 M NaCl containing 0.02% thimerosal
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Elizabeth Head et al.
Journal of Alzheimer's disease : JAD, 20(2), 637-646 (2010-02-19)
The study of Alzheimer's disease (AD) pathogenesis requires the use of animal models that develop some amount of amyloid pathology in the brain. Aged canines (beagles) naturally accumulate human-type amyloid-beta peptide (Abeta) and develop parallel declines in cognitive function. However
K Islam et al.
Analytical biochemistry, 137(1), 8-14 (1984-02-01)
A procedure for the rapid purification of nucleoside diphosphate kinase, 24 h with a single operator, from the chick brain soluble fraction is described. The influence of the ionic conditions on the association-disassociation properties of the enzyme are exploited to
S R Narayanan et al.
Biochimica et biophysica acta, 965(1), 22-28 (1988-04-14)
S-Adenosylhomocysteine (AdoHcy) hydrolase (adenosylhomocysteinase, EC 3.3.1.1) was purified from bovine liver by conventional protein purification procedures (differential centrifugation, ammonium sulfate fractionation and DEAE-cellulose chromatography) followed by affinity chromatography on blue dextran coupled to agarose. The enzyme was eluted from the
Anja Maria Pieslinger et al.
The Journal of biological chemistry, 285(5), 2902-2910 (2009-12-03)
Nucleotide sugars are building blocks for carbohydrate polymers in plant cell walls. They are synthesized from sugar-1-phosphates or epimerized as nucleotide sugars. The main precursor for primary cell walls is UDP-glucuronic acid, which can be synthesized via two independent pathways.
K R Smith et al.
The Journal of biological chemistry, 264(11), 6119-6126 (1989-04-15)
A polypeptide (Mr = 15,000) has been purified from Escherichia coli cell extracts that significantly stimulates the duplex DNA unwinding reaction catalyzed by E. coli Rep protein. The Rep helicase unwinding reaction was stimulated by as much as 20-fold, upon
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