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Merck
CN

B6274

5-Bromo-4-chloro-3-indolyl phosphate dipotassium salt

phosphatase substrate

Synonym(s):

BCIP®, X-phosphate dipotassium salt

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About This Item

Linear Formula:
C8H4BrClNO4PK2
CAS Number:
102185-49-9
Molecular Weight:
402.65
NACRES:
NA.83
PubChem Substance ID:
329773274
UNSPSC Code:
12352204
MDL number:
MFCD00080893

Key Documents

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InChI

1S/C8H6BrClNO4P.K.H/c9-4-1-2-5-7(8(4)10)6(3-11-5)15-16(12,13)14;;/h1-3,11H,(H2,12,13,14);;

SMILES string

[K].OP(O)(=O)Oc1c[nH]c2ccc(Br)c(Cl)c12

InChI key

HPKPHSVUJYUSDD-UHFFFAOYSA-N

assay

≥99% (HPLC)

form

powder

solubility

water: 50 mg/mL, clear to hazy

storage temp.

−20°C

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General description

Histochemical substrate for alkaline phosphatase.

Legal Information

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLBV8400
120M5212
BGBB3257V
BGBB3257
027K5001

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Anne Jørgensen et al.
Reproductive biology and endocrinology : RB&E, 7, 97-97 (2009-09-15)
Investigating gonadal gene expression is important in attempting to elucidate the molecular mechanism of sex determination and differentiation in the model species zebrafish. However, the small size of juvenile zebrafish and correspondingly their gonads complicates this type of investigation. Furthermore
Si Brask Sonne et al.
PloS one, 4(5), e5536-e5536 (2009-05-14)
Microarray and RT-PCR based methods are important tools for analysis of gene expression; however, in tissues containing many different cells types, such as the testis, characterization of gene expression in specific cell types can be severely hampered by noise from
Daniel Bröker et al.
Applied and environmental microbiology, 74(8), 2288-2297 (2008-02-26)
The latex-clearing protein (Lcp(K30)) from the rubber-degrading bacterium Streptomyces sp. strain K30 is involved in the cleavage of poly(cis-1,4-isoprene), yielding isoprenoid aldehydes and ketones. Lcp homologues have so far been detected in all investigated clearing-zone-forming rubber-degrading bacteria. Internal degenerated oligonucleotides
Zong-Keng Kuo et al.
Diagnostics (Basel, Switzerland), 9(4) (2019-11-30)
Detecting small amounts of analyte in clinical practice is challenging because of deficiencies in specimen sample availability and unsuitable sampling environments that prevent reliable sampling. Paper-based analytical devices (PADs) have successfully been used to detect ultralow amounts of analyte, and

Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

比色碱性磷酸酶和过氧化物酶底物检测系统

硝基蓝四唑（NBT）可与碱性磷酸酶底物5-溴-4-氯-3-吲哚基磷酸盐（BCIP）一起用于蛋白印迹和免疫组织染色程序。这些底物系统产生不溶的NBT二甲终产物，其颜色为蓝至紫色，可以通过视觉观察到。

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