B8291
BSK-H Medium, Complete
With 6% rabbit serum, liquid, sterile-filtered, suitable for cell culture
Synonym(s):
Barbour-Stoenner-Kelly medium, Borrelia burgdorferi medium
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About This Item
UNSPSC Code:
12352207
NACRES:
NA.75
Product Name
BSK-H Medium, Complete, sterile-filtered, with 6% rabbit serum, suitable for Borrelia burgdorferi
sterility
sterile-filtered
form
liquid
suitability
suitable for Borrelia burgdorferi
application(s)
food and beverages
components
NaHCO3: 2.068 g/L
phenol red: 0.01997 g/L
glucose: 5.64 g/L (Dextro)
HEPES: 5.64 g/L
shipped in
dry ice
storage temp.
−20°C
Quality Level
Application
Complete BSK-H Medium was used to culture B. burgdorferi to study the pathology of lyme borreliosis. It may be used in serum supplemented formulation for the growth and expansion of the Lyme desease spirochete, Borrelia burgdorferi.
Biochem/physiol Actions
BSK-H medium is a standardized complex medium designed to support the growth of the Lyme disease spirochete, Borrelia burgdorferi. To standardize the procedure for isolating and culturing Lyme disease spirochetes, Pollack, RJ et. al. modified the composition of the medium generally used for this purpose (BSK-II) and developed a system for its distribution. This medium contains no gelatin or agarose, and various components are used in proportions that differ from those in BSK-II. Each of the major proteinacious components was screened by substitution in samples of the complete product. The final medium was evaluated for the capacity to grow related spirochetes including Borrelia burgdorferi N40, Guilford, and JD-1 as well as strains of Borrelia hermsii (HS-1) and of Borrelia coriaceae (CO53). Standardized medium, supplemented with prescreened rabbit serum, facilitates comparison of research results between laboratories and may eventually permit definitive clinical diagnosis of Lyme disease based on demonstration of the pathogen. The standardized medium is designated BSK-H.
Barbour-Stonner-Kelly (BSK) medium is a modified medium for the culture of Borrelia strains that cause lyme borreliosis. BSK medium is a cheaper alternative for the selective culture of B. burgdorferi s.l. strains but not B. afzelii strain.
Barbour-Stonner-Kelly (BSK) medium is a modified medium for the culture of Borrelia strains that cause lyme borreliosis. BSK medium is a cheaper alternative for the selective culture of B. burgdorferi s.l. strains but not B. afzelii strain.
Preparation Note
BSK-H Medium is supplied as a sterile-filtered liquid. After thawing, mix well by inverting the bottle prior to use. If the thawed medium will not be used within a few days it is recommended that the medium be refrozen in working aliquots to avoid repeated free-thaw cycles. Other supplements may be added aseptically as desired. The nature of the supplement may affect storage conditions and shelf-life of the medium.
Storage Class
12 - Non Combustible Liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Regulatory Information
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Nataliia Rudenko et al.
Journal of clinical microbiology, 46(10), 3540-3543 (2008-07-25)
Molecular analysis of a clinical sample confirmed the presence of Borrelia bissettii DNA in cardiac valve tissue from a patient with endocarditis and aortic valve stenosis. This evidence strongly supports the involvement of B. bissettii in Lyme disease in Europe.
Zachary A Schiller et al.
The Journal of clinical investigation, 131(11) (2021-04-30)
Disrupting transmission of Borrelia burgdorferi sensu lato complex (B. burgdorferi) from infected ticks to humans is one strategy to prevent the significant morbidity from Lyme disease. We have previously shown that an anti-OspA human mAb, 2217, prevents transmission of B.
Jana Tuhácková et al.
Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia, 149(2), 297-302 (2006-04-08)
Verification of the efficacy of Biocan B inj. ad us. vet. (Bioveta, a.s.) was done by challenge testing. Ticks collected in the nature were used as natural vectors of the infection. Six beagles and two control ones were used in
Deborah A Grosenbaugh et al.
BMC veterinary research, 14(1), 312-312 (2018-10-18)
Prevention of Lyme disease in dogs in North America depends on effective vaccination against infection by the tick vector-born spirochete Borrelia burgdorferi. Most vaccines effectively prevent spirochete transmission to dogs during tick feeding based on immunization with the outer-surface lipoprotein
Sin Hang Lee et al.
American journal of clinical pathology, 133(4), 569-576 (2010-03-17)
The DNA of Borrelia burgdorferi spirochetes extracted by ammonium hydroxide was used as the template for nested polymerase chain reaction (PCR) amplification of the species-specific 16S ribosomal DNA (rDNA). The primers were those well known to be specific for signature
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