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BX-0100-30

Human Spinal Motor Neurons

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Human iPSC line, Fully differentiated, Healthy Male Control (no known neurological disorders), Cryopreserved

Synonym(s):

Lower Motor Neurons

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biological source

human (iPSC line)

form

frozen liquid

packaging

vial of 1 (contains ≥1 million cells)
vial of 1 (contains ≥5 million cells)

technique(s)

cell culture | mammalian: suitable

storage temp.

-140 to -196°C

Related Categories

General description

Human iPSC-Derived Spinal Motor Neurons. GFP-expressing neurons also available (BX-0101-30-1M). Required supplements for the final cell differentiation and maturation (sold separately): BX-2100-100uL, BX-2020-100uL, BX-2040-100uL. Located in the spinal cord or at the brain stem within the motor cortex, motor neurons indirectly or directly carry signals to muscles, organs, or glands from the brain. These neurons have one axon with multiple dendrites, and can be classified either as upper motor neurons, which signal between the brain and the spinal cord, or lower motor neurons, which carry signals to muscles from the spinal cord. Related diseases: ALS, SMA, Spinal Cord Injury. Primary neurotransmitter released: Acetylcholine.

Application

Function: Spinal Motor Neurons are adherent and exhibit substantial neurite outgrowth within the first week in culture. Spinal Motor Neurons exhibit pronounced electrophysiological activity after two weeks in culture, as demonstrated by multi-electrode array (MEA) recordings. Calcein staining demonstrates the elaborate processes of the Spinal Motor Neurons in culture.

Features and Benefits

Marker Expression: Fully differentiated Spinal Motor Neurons have high neuronal purity (>90%) and consist of greater than 70% motor neurons. Labeling with the pan-neuronal marker MAP2 (green) and the motor neuron-specific marker FOXP1 (red) highlight the purity of this neuronal product.

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James C Dodge et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 40(47), 9137-9147 (2020-10-15)
Amyotrophic lateral sclerosis (ALS) is a fatal neuromuscular disease characterized by motor neuron (MN) death. Lipid dysregulation manifests during disease; however, it is unclear whether lipid homeostasis is adversely affected in the in the spinal cord gray matter (GM), and
Lu-Lin Jiang et al.
The Journal of clinical investigation, 129(8), 3103-3120 (2019-05-22)
Mechanisms underlying motor neuron degeneration in amyotrophic lateral sclerosis (ALS) are yet unclear. Specific deletion of the ER-component membralin in astrocytes manifested postnatal motor defects and lethality in mice, causing the accumulation of extracellular glutamate through reducing the glutamate transporter

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