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Merck
CN

C0354

Sigma-Aldrich

Anti-p130CAS antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

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About This Item

MDL number:
MFCD01633943
UNSPSC Code:
12352203

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biological source

rabbit

conjugate

unconjugated

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 130 kDa

species reactivity

mouse, human, rat

technique(s)

immunoprecipitation (IP): 3 μg using using Protein A-agarose and 10 μg lysate of cultured ECV304 human endothelial cells or mouse fibroblasts NIH3T3 cell lysate
microarray: suitable
western blot: 1:1,000 using whole extract of the human ECV304 endothelial cells or a whole extract of the rat skeletal muscle myoblasts L8 cell line

UniProt accession no.

P56945

storage temp.

−20°C

Gene Information

human ... BCAR1(9564)
mouse ... Bcar1(12927)
rat ... Bcar1(25414)

General description

p130cas or CAS (Crk-associated substrate) is a 120-130kD docking protein consists of a repetitive stretch of tyrosine-containing motifs. It has a pivotal role in cell adhesion, cell migration, cytokine receptor engagement and growth factor stimulation. It also has a critical role in cell transformation. Anti-(p130cas) antibody can be used in western blotting (diluted 1:1000) using human ECV304 endothelial cell extracts and rat skeletal muscle myoblasts L8 cell line extract. Rabbit anti-(p130cas) antibody reacts specifically with p130cas of rat, mouse and human.

Immunogen

Synthetic peptide corresponding to the C-terminal region of human (p130cas) (amino acids 642-659), coupled to KLH. This sequence is identical in mouse and rat (p130cas) and has no homology to Cas-related proteins Hef1/Cas-L and Efs/Sin.

Application

Anti-(p130cas) antibody can be used in immunoprecipitation and microarray.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

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Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
111M4835
070K4837

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Differential regulation of cell migration, actin stress fiber organization, and cell transformation by functional domains of Crk-associated substrate.
Huang J, Hamasaki H
Biological Chemistry, 277(30), 27265-27272 (2002)
Fariborz Nasertorabi et al.
Biochemical and biophysical research communications, 324(3), 993-998 (2004-10-16)
The docking protein p130Cas becomes phosphorylated upon cell adhesion to extracellular matrix proteins, and is thought to play an essential role in cell transformation. Cas transmits signals through interactions with the Src-homology 3 (SH3) and Src-homology 2 domains of FAK

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