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Merck
CN

C0646

Cytodex® 1 microcarrier beads

Cytodex 1, 60-87 μm

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About This Item

CAS Number:
UNSPSC Code:
41121800
NACRES:
NB.22
MDL number:
Technical Service
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material

dextran beads

sterility

non-sterile

particle size

60-87 μm

density

1.03 g/cm3 at 25 °C

General description

A general-purpose microcarrier for most established, anchorage-dependent cell lines.

High yield culture of anchorage-dependent cells: Microcarrier culture is a technique which makes possible the practical high yield culture of anchorage-dependent cells. Cytodex has been specifically developed for the culture of a wide range of animal cells in culture volumes ranging from a few mL to more than 6000 L. Using Cytodex in simple suspension culture systems provides yields of several million cells per mL.

Suitable for most established cell lines: Formed by substituting a cross-linked dextran matrix with positively charged DEAE* groups distributed throughout the matrix, is a general purpose microcarrier. It is particularly suitable for most established cell lines and for production of viruses or cell products from cultures of primary cells and normal diploid cell strains.

Application

For general microcarrier culture.

The particle size of this product averages 190um when swelled in liquid solutions (as in use). The particle size of 60-87 um relates to the beads in dry form.

Features and Benefits

  • General-purpose microcarrier for most established, anchorage-dependent cell lines.
  • Contains positive charges throughout the microporous matrix.
  • Transparent for easy microscopic examination of attached cells.

Preparation Note

Prior to use, swell beads in PBS (50 mL/g) and autoclave at 120 °C.

Other Notes

Dextran beads with positive-charged DEAE groups throughout matrix.

Legal Information

Cytodex is a registered trademark of Cytiva


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Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Martin Wurm et al.
Tissue engineering. Part A, 15(5), 1063-1073 (2008-10-14)
The aim of this study was to establish a stand-alone, perfused, rotary cell culture system using small human hepatocytes (SH) for bioartificial liver (BAL) support. SH were grown on cytodex 3 microcarriers (beads) to a maximum density of 1.2 +/-
Xu Tao et al.
Journal of biomedical materials research. Part A, 65(2), 306-310 (2003-05-08)
Porous gelatin microcarriers having a diameter of 80-100 microm were prepared by the suspension method using toluene as the oil phase. Rat hepatocytes were cultured on gelatin and cytodexIII microcarriers. The cells retained its spherical shape, which is similar in
Quanyu Zhao et al.
Biotechnology progress, 19(5), 1569-1573 (2003-10-04)
Toward the development of an in vitro cultivation of marine sponge cells for sustainable production of bioactive metabolites, the attachment characteristics of marine sponge cells of Hymeniacidon perleve on three types of microcarriers, Hillex, Cytodex 3, and glass beads, were



Global Trade Item Number

SKUGTIN
C0646-5G04061832659626
C0646-100G04061832659602
C0646-25G04061832659619