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Merck
CN

C0646

Cytodex® 1 microcarrier beads

Cytodex 1, 60-87 μm

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About This Item

CAS Number:
UNSPSC Code:
41121800
NACRES:
NB.22
MDL number:
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Product Name

Cytodex® 1 microcarrier beads, Cytodex 1, 60-87 μm

material

dextran beads

sterility

non-sterile

particle size

60-87 μm

density

1.03 g/cm3 at 25 °C

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Application

For general microcarrier culture.

The particle size of this product averages 190um when swelled in liquid solutions (as in use). The particle size of 60-87 um relates to the beads in dry form.

Features and Benefits

  • General-purpose microcarrier for most established, anchorage-dependent cell lines.
  • Contains positive charges throughout the microporous matrix.
  • Transparent for easy microscopic examination of attached cells.

General description

A general-purpose microcarrier for most established, anchorage-dependent cell lines.

High yield culture of anchorage-dependent cells: Microcarrier culture is a technique which makes possible the practical high yield culture of anchorage-dependent cells. Cytodex has been specifically developed for the culture of a wide range of animal cells in culture volumes ranging from a few mL to more than 6000 L. Using Cytodex in simple suspension culture systems provides yields of several million cells per mL.

Suitable for most established cell lines: Formed by substituting a cross-linked dextran matrix with positively charged DEAE* groups distributed throughout the matrix, is a general purpose microcarrier. It is particularly suitable for most established cell lines and for production of viruses or cell products from cultures of primary cells and normal diploid cell strains.

Other Notes

Dextran beads with positive-charged DEAE groups throughout matrix.

Preparation Note

Prior to use, swell beads in PBS (50 mL/g) and autoclave at 120 °C.

Legal Information

Cytodex is a registered trademark of Cytiva

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Christopher J Hewitt et al.
Biotechnology letters, 33(11), 2325-2335 (2011-07-20)
The effects on human mesenchymal stem cell growth of choosing either of two spinner flask impeller geometries, two microcarrier concentrations and two cell concentrations (seeding densities) were investigated. Cytodex 3 microcarriers were not damaged when held at the minimum speed
Kamla Dutt et al.
Current stem cell research & therapy, 6(4), 350-361 (2011-04-07)
Retinal degenerations are the leading cause of genetically inherited blindness. One of the strategies currently being tested for the treatment is cell/tissue transplantation. As such stem cells and tissue engineered constructs are of great importance. This report describes the growth
Elsa Abranches et al.
Biotechnology and bioengineering, 96(6), 1211-1221 (2006-09-29)
Embryonic stem (ES) cells have been shown to differentiate in vitro into a wide variety of cell types having significant potential for tissue regeneration. Therefore, the operational conditions for the ex vivo expansion and differentiation should be optimized for large-scale
Jan Cedergren et al.
The Journal of rheumatology, 34(11), 2162-2170 (2007-10-17)
To compare total and intracellular oxidative activation of blood and synovial fluid (SF) neutrophils from patients with rheumatoid arthritis (RA) and other arthritides with blood donor neutrophils. Peripheral blood and SF samples were obtained from 26 gonarthritis patients (13 RA
A M Fernandes et al.
Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas, 42(6), 515-522 (2009-05-19)
Future clinical applications of human embryonic stem (hES) cells will require high-yield culture protocols. Currently, hES cells are mainly cultured in static tissue plates, which offer a limited surface and require repeated sub-culturing. Here we describe a stirred system with

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