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C0794

Chitosanase from Streptomyces sp.

buffered aqueous glycerol solution, ≥15 units/mg protein (E1%)

Synonym(s):

Chitosan N-acetylglucosaminohydrolase

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About This Item

CAS Number:
51570-20-8
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
3.2.1.132 (BRENDA, IUBMB)
MDL number:
MFCD00211797
Specific activity:
≥15 units/mg protein (E1%)
Concentration:
≥0.5 mg/mL
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form

buffered aqueous glycerol solution

Quality Segment

200

specific activity

≥15 units/mg protein (E1%)

concentration

≥0.5 mg/mL

solubility

0.1 M acetate buffer, pH 5.5: soluble

storage temp.

−20°C

Application

Chitosanase from Sigma has been used for polarized light-stimulated, enzymatic hydrolysis of chitin and chitosan.

Biochem/physiol Actions

Chitosanase catalyzes the endohydrolysis of β-1,4-linkages between D-glucosamine (GlcN-GlcN) residues in chitosan. The enzyme from Streptomyces has been reported to also hydrolyze the GlcNAc-GlcN linkage in partially acetylated chitosan.

Physical form

Solution in 50% glycerol containing 50 mM sodium acetate

Preparation Note

Soluble in 10mL of 0.1 M Acetate buffer at pH 5.5

Other Notes

One unit will liberate 1.0 μmole of reducing sugar (measured as D-glucosamine equivalents) from chitosan per minute at pH 5.5 at 37 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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