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C1261

Carboxypeptidase A−Agarose

Name: Carboxypeptidase A−Agarose
Brand: SIGMA

ammonium sulfate suspension, ≥6 units/mL packed gel, 25 °C, enzyme from bovine pancreas

Synonym(s):

Carboxypolypeptidase, Peptidyl-L-amino-acid hydrolase

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.54

EC Number:

3.4.17.1 (BRENDA, IUBMB)

MDL number:

MFCD00130718

Specific activity:

≥6 units/mL packed gel, 25 °C

Biological source:

enzyme from bovine pancreas

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biological source

enzyme from bovine pancreas

Quality Level

200

form

ammonium sulfate suspension

specific activity

≥6 units/mL packed gel, 25 °C

mol wt

~35,250

matrix

beaded agarose

storage temp.

2-8°C

Related Categories

Proteases

Proteins & Enzymes

General description

Carboxypeptidase A-agarose product is prepared by the immobilization of carboxypeptidase A, originally isolated from the bovine pancreas, to activated 4% crosslinked beaded agarose.

Biochem/physiol Actions

Carboxypeptidase A is attached covalently to agarose or aldehyde and is effective for immobilization studies.

Carboxypeptidase as isolated from bovine pancreas glands is a metalloenzyme that contains 1 g atom of zinc per mole of protein. It catalyzes the hydrolysis of the carboxyl-terminal peptide bond in peptides and proteins. It is primarily specific to aromatic and hydrophobic side chains such as phenylalanine, tryptophan or leucine. The enzyme also exhibits esterase activity. It is inhibited by β-phenylpropionate and indole acetate.

Physical form

Suspension in 2.0 M (NH₄)₂SO₄, pH 7

Other Notes

One unit will hydrolyze 1.0 μmole of hippuryl-L-phenylalanine per min at pH 7.5 at 25 °C.

pictograms

GHS08,GHS07

signalword

Danger

hcodes

H315,H319,H334,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

10 - Combustible liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

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Stabilization-immobilization of carboxypeptidase A to aldehyde-agarose gels: A practical example in the hydrolysis of casein

Pedroche J, et al.

Enzyme and Microbial Technology, 711-718 null

Isolation of Metarhizium anisopliae carboxypeptidase A with native disulfide bonds from the cytosol of Escherichia coli BL21(DE3).

Brian P Austin et al.

Protein expression and purification, 82(1), 116-124 (2011-12-27)

The carboxypeptidase A enzyme from Metarhizium anisopliae (MeCPA) has broader specificity than the mammalian A-type carboxypeptidases, making it a more useful reagent for the removal of short affinity tags and disordered residues from the C-termini of recombinant proteins. When secreted

Deleting Mcl-1 in mast cells: getting 2 birds with 1 stone.

Booki Min

Blood, 118(26), 6729-6730 (2011-12-24)

Confirmation of a potential biomarker for early-stage pancreatic cancer.

Arthur A Topilow et al.

Cancer biomarkers : section A of Disease markers, 10(1), 27-33 (2012-02-03)

Pancreatic cancer has a dismal prognosis because it is often diagnosed at an advanced stage. Therefore, serological biomarkers are eagerly sought for early detection. The digestive enzyme pro-carboxypeptidase A (PCPA) may be able to fill this role. The purpose of

Identification of two-histidines one-carboxylate binding motifs in proteins amenable to facial coordination to metals.

Beat Amrein et al.

Metallomics : integrated biometal science, 4(4), 379-388 (2012-03-07)

Among natural metalloenzymes, the facial two-histidines one-carboxylate binding motif (FTM) is a widely represented first coordination sphere motif present in the active site of a variety of metalloenzymes. A PDB search revealed a total of 1685 structures bearing such FTMs

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Protocols

Enzymatic Assay: Carboxypeptidase

Carboxypeptidase A activity measured via continuous spectrophotometric rate determination assay with hippuryl-L-phenylalanine substrate.

羧肽酶A酶活检测

在测定羧肽酶A活性时，使用马尿酸-L-苯丙氨酸在254nm处进行连续分光光度法测定。羧肽酶A水解C末端残基上的肽键。

Global Trade Item Number

SKU	GTIN
C1261-10UN	04061832699875
C1261-25UN	04061832699882

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