C1809
Collagen from kangaroo tail
Bornstein and Traub Type I (Sigma Type I), powder, suitable for cell culture
form
powder
technique(s)
cell culture | mammalian: suitable
surface coverage
6‑10 μg/cm2
solubility
aqueous acid: soluble (1 - 2μl acetic acid per ml H2O)
storage temp.
2-8°C
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General description
Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.
Application
This collagen has been tested in culture with mammalian cells to verify it is low in endotoxin content. Type I collagen is a component of skin, bone, tendon, and other fibrous connective tissues. It is often used in cell culture as an attachment substratum. Myoblasts, spinal ganglia, hepatocytes, embryonic lung, heart explants, fibroblasts, endothelial cells, and islet cells have been cultured successfully on films or gels of Type I collagen. Type I collagen differs from other collagens by their low lysine hydroxylation and low carbohydrate composition.
Physical form
Lyophilized from 10 mM glacial acetic acid
Preparation Note
Prepared by a modification of the pepsin extraction method of Niyibizi, et al., J. Biol. Chem., 259, 14170 (1984).
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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C Niyibizi et al.
The Journal of biological chemistry, 259(22), 14170-14174 (1984-11-25)
Human type V collagen was purified from placenta and found to contain alpha 1(V), alpha 2(V), and alpha 3(V) chains in varying ratios. Using any of three independent nondenaturing methods (phosphocellulose chromatography, high-performance ion-exchange chromatography on IEX-540 DEAE, and ammonium
Simon Holland et al.
Current opinion in ophthalmology, 24(4), 302-309 (2013-05-18)
Topography-guided laser refractive surgery regularizes the front corneal surface irregularities to achieve the desired refractive outcome. This is particularly applicable in highly aberrated corneas, where wavefront aberrometry is often not possible. This article aims to review the recently published results
Visian toric ICL implantation for residual refractive errors after ICRS implantation and corneal collagen cross-linking in keratoconus.
Elias Jarade et al.
Journal of refractive surgery (Thorofare, N.J. : 1995), 29(7), 444-444 (2013-07-04)
A B Shori et al.
Pakistan journal of biological sciences : PJBS, 15(24), 1160-1167 (2013-06-13)
There is an increasing demand of functional foods in developed countries. Yogurt plays an important role in the management of blood pressure. Several bioactive peptides isolated from Allium sativum or fish collagen have shown antihypertensive activity. Thus, in the present
Akiko Nagaishi et al.
Nihon rinsho. Japanese journal of clinical medicine, 71(5), 876-880 (2013-06-20)
Patients with myasthenia gravis(MG) are divided into three groups: (1) acetylcholine receptor antibody positive MG: 80%, (2) muscle-specific receptor tyrosine kinase (MuSK) antibody positive MG: 5-10%, and (3) double seronegative MG. In 2011, autoantibodies (Abs) against low-density lipoprotein receptor-related protein
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