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Merck
CN

C1897

Sigma-Aldrich

Catechol-O-methyl Transferase from porcine liver

lyophilized powder, ≥150 units/mg protein

Synonym(s):

COMT, Pyrocatechol-O-methyl Transferase, S-Adenosyl-L-methionine:catechol O-methyltransferase

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About This Item

CAS Number:
EC Number:
EC Number:
MDL number:
UNSPSC Code:
12352200
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form

lyophilized powder

specific activity

≥150 units/mg protein

purified by

chromatography

composition

Protein, 30-60% biuret

storage temp.

−20°C

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Biochem/physiol Actions

Major degradative enzyme for catecholamine neurotransmitters; methylates the o-hydroxyl group

Physical form

Lyophilized powder containing phosphate buffer and dithiothreitol

Other Notes

One unit will produce 1.0 nanomole of total O-methylated products from 3,4 dihydroxyacetophenone per hour at pH 7.6 at 37 °C.
Previous radioactive assay unit definition: One unit will catalyze the methylation of 1.0 nanomole of protocatechuic acid per hr at pH 7.9 at 37°C using S-adenosyl-L-[methyl-14C]methionine as the methyl donor. (~6 radioactive units equal one new unit).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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M C Wardle et al.
Genes, brain, and behavior, 12(1), 13-20 (2012-12-13)
In a widely cited study, Mattay et al. reported that amphetamine (0.25 mg/kg oral, or 17 mg for a 68 kg individual) impaired behavioral and brain indices of executive functioning, measured using the Wisconsin Card Sorting Task (WCST) and N-Back
Nidhi Jatana et al.
Journal of molecular graphics & modelling, 39, 145-164 (2013-01-03)
Catechol-O-methyltransferase (COMT) catalyzes the methylation of catecholamines, including neurotransmitters like dopamine, epinephrine and norepinephrine, leading to their degradation. COMT has been a subject of study for its implications in numerous neurological disorders like Parkinson's disease (PD), schizophrenia, and depression. The
Excessive daytime sleepiness and its risk factors in incident Parkinson's disease.
David P Breen et al.
Journal of neurology, neurosurgery, and psychiatry, 84(2), 233-234 (2012-11-28)
Luis Almeida et al.
Clinical pharmacokinetics, 52(2), 139-151 (2012-12-19)
Opicapone is a novel catechol-O-methyltransferase (COMT) inhibitor. The purpose of this study was to evaluate the tolerability, pharmacokinetics (including the effect of food) and pharmacodynamics (effect on COMT activity) following single oral doses of opicapone in young healthy male volunteers.
Ruth Landau et al.
Anesthesia and analgesia, 116(2), 386-391 (2013-01-11)
IV fentanyl is used as a labor analgesic; however, few studies have reported the effects of IV fentanyl for early labor analgesia. We evaluated the analgesic response to IV fentanyl according to the combined effect of the single-nucleotide polymorphisms rs1799971

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