C2026
Ceruloplasmin bovine
2,000-6,000 units/mL, saline solution, oxidase 20-60 units/mg protein
form
saline solution
oxidase activity
20-60 units/mg protein
mol wt
~135 kDa
concentration
2,000-6,000 units/mL
copper content
≥80 μg/mL
UniProt accession no.
storage temp.
2-8°C
Gene Information
cow ... CP(280681)
Biochem/physiol Actions
Ceruloplasmin (Cp), the principal carrier of copper in plasma, is thought to be involved in oxidative modification of lipoproteins and participate in the acute phase reaction to stress. Cp is an effective antioxidant for a variety of radicals and has a potent peroxidase activity to decompose hydrogen peroxide in the presence of reduced glutathione. It inhibits the peroxidase activity of myeloperoxidase in a concentration-dependent manner and shows selective binding to myeloperoxidase in vitro. This suggests that ceruloplasmin may participate in the clearance and inactivation of myeloperoxidase in vivo. At physiological (micromolar) concentrations it was found to inhibit relaxation of rabbit aorta induced by endothelium-dependent agonists like acetylcholine or ADP. However, it was found ineffective toward vasodilation due to direct stimulation of smooth muscle cells by nitroglycerin.
Ceruloplasmin (Cp), the principal carrier of copper in plasma, is thought to be involved in oxidative modification of lipoproteins and participate in the acute phase reaction to stress. Cp is an effective antioxidant for a variety of radicals and has a potent peroxidase activity to decompose hydrogen peroxide in the presence of reduced glutathione. It inhibits the peroxidase activity of myeloperoxidase in a concentration-dependent manner and shows selective binding to myeloperoxidase in vitro. This suggests that ceruloplasmin may participate in the clearance and inactivation of myeloperoxidase in vivo. At physiological (micromolar) concentrations it was found to inhibit relaxation of rabbit aorta induced by endothelium-dependent agonists like acetylcholine or ADP. However, it was found ineffective toward vasodilation due to direct stimulation of smooth muscle cells by nitroglycerin. Cp plays an essential role in iron homeostasis, regulating the efflux of iron from macrophages and the oxidation of Fe2+ to Fe3+. Cp deficiency leads to neurodegenerative disorders, possibly by starving neurons of iron.
Physical form
Solution in 0.25 M sodium chloride, 0.05 M sodium acetate, pH ~7.0
Analysis Note
Protein determined by biuret
Other Notes
One unit will cause a ΔA550 of 0.01 per min using N,N-dimethyl-p-phenylenediamine as substrate at pH 6.4 and 37 °C, in a 7 mL reaction volume.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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The pH optimum for ceruloplasmin oxidase activity in the plasma of several species of animal.
J B Bingley et al.
Clinica chimica acta; international journal of clinical chemistry, 25(3), 480-482 (1969-09-01)
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