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Key Documents

COO/COA

C2372

Anti-CSTF1 (C-terminal) antibody produced in rabbit

Name: Anti-CSTF1 (C-terminal) antibody produced in rabbit
Brand: SIGMA

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):

Anti-Cleavage stimulation factor, 3-prime PRE-RNA, subunit 1, 50-KD, Anti-CstF-50, Anti-CstFp50

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

biological source

rabbit

Quality Level

200

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen ~48 kDa

species reactivity

human

concentration

~1 mg/mL

technique(s)

immunoprecipitation (IP): 5-10 μg using K562 lysates
western blot: 2-4 μg/mL using K562 lysates

UniProt accession no.

Q05048

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CSTF1(1477)
mouse ... Cstf1(67337)
rat ... Cstf1(311670)

General description

Cleavage stimulation factor 1 (CSTF1) contains seven WD-40 repeats. The CSTF1 gene is mapped to human chromosome 20q13.2-q13.31.

Specificity

Anti-CSTF1 (C-terminal) specifically recognizes human CSTF1 (also known as Cleavage stimulation factor).

Application

Anti-CSTF1 (C-terminal) antibody produced in rabbit is suitable for immunoprecipitation at a working amount of 5-10μg using K562 lysates and western blotting at a concentration of 2-4μg/mL using K562 lysates.

Biochem/physiol Actions

CSTF1 is required for in vitro cleavage activity. The WD-40 repeats are crucial for the interaction with CSTF3. CSTF1 also interacts directly with the RNA polymerase II CTD, suggesting a role in linking transcription and mRNA 3′-ends processing.

Cleavage stimulation factor (50kDa) subunit is a protein encoded by the CSTF1 gene in humans. It is one of the multiple factors that plays a significant role during polyadenylation of mammalian pre-mRNAs. The mRNA polyadenylation factor CstF interacts with the BRCA1-associated protein BARD1 resulting in repression of the nuclear mRNA polyadenylation machinery. It participates in the regulation of endogenous transcripts during DNA-damage.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Certificates of Analysis (COA)

Lot/Batch Number

079K4758

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