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Merck
CN

C2898

Sigma-Aldrich

Choline Acetyltransferase from human placenta

suitable for assay of choline, ammonium sulfate suspension, ≥20 units/mg protein (biuret)

Synonym(s):

AChT, Acetyl-CoA: choline O-acetyltransferase, CAT

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC Code:
12352200
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form

ammonium sulfate suspension

specific activity

≥20 units/mg protein (biuret)

suitability

suitable for assay of choline

UniProt accession no.

storage temp.

2-8°C

Gene Information

human ... CHAT(1103)

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Biochem/physiol Actions

Choline acetyltransferase (AChT) is a major enzyme in the synthesis of acetylcholine (ACh) in cholinergic neurons.

Physical form

Suspension in 2.8 M (NH4)2SO4 containing 5 mM potassium phosphate, pH 7.4, 0.5 mM EDTA and 0.5 mM dithiothreitol

Analysis Note

Actual protein content given on label.

Other Notes

One unit will catalyze the transfer of 1.0 nanomole of acetate from 14C-acetyl CoA to choline per min at pH 7.5 at 37°C.

Regulatory Information

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M Zalecki et al.
Autonomic neuroscience : basic & clinical, 171(1-2), 21-27 (2012-10-30)
The goal of the present study was to examine the precise localization of the brainstem motor and primary sensory (nodose ganglion) vagal perikarya supplying the pylorus in the domestic pig. Using the Fast Blue retrograde tracing technique it has been
N Kayadjanian et al.
The European journal of neuroscience, 11(10), 3659-3668 (1999-11-17)
The regulation of the striatal m1 and m4 muscarinic receptor mRNA as well as the choline acetyltransferase (ChAT) mRNA expression by nigral dopaminergic and cortical glutamatergic afferent fibres was investigated using quantitative in situ hybridization histochemistry. The effects induced by
Dale F Sharrad et al.
Neuroscience letters, 534, 171-176 (2012-11-06)
The discrete functional classes of enteric neurons in the mammalian gastrointestinal tract have been successfully distinguished on the basis of the unique combination of molecules and enzymes in their cell bodies ("chemical coding"). Whether the same chemical coding exists in
Li Xu et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 37(19), 2943-2946 (2012-12-29)
To study the effect of Sailuotong capsule (Sailuotong) on learning and memory functions of multi-infarct dementia (MID) rats and its mechanism. All SD rats were divided into five groups, namely the sham operation group, the model group, the positive group
F Mignini et al.
Lymphatic research and biology, 10(4), 189-197 (2012-12-18)
The present work deals with innervation patterns along collector lymphatic vessels from cervical, mesenteric, and femoral regions, and lymph capillaries in young and elderly subjects. Morphological and morphometric analysis of nerve fibers along lymph vessels was performed by immunohistochemistry for

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