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Merck
CN

C2900

Chloramphenicol Acetyltransferase from Escherichia coli

lyophilized powder, 50,000-150,000 units/mg protein (Lowry)

Synonym(s):

Acetyl-CoA: chloramphenicol 3-O-acetyltransferase, CAT

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About This Item

CAS Number:
9040-07-7
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
2.3.1.28 (BRENDA, IUBMB)
MDL number:
MFCD00130772
Specific activity:
50,000-150,000 units/mg protein (Lowry)

Key Documents

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form

lyophilized powder

specific activity

50,000-150,000 units/mg protein (Lowry)

mol wt

75 kDa (three identical subunits)

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Gene Information

Escherichia coli ... cat(2847485)

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General description

The enzyme has a molecular weight of approximately 78,000 Da and pH optimum of 7.8.

Biochem/physiol Actions

The enzyme responsible for chloramphenicol resistance in bacteria. Catalyzes the conversion of acetyl-CoA + chloramphenicol to CoA + chloramphenicol 3-acetate.

Physical form

Partially purified; contains Tris buffer salts.

Other Notes

One unit will convert 1.0 nanomole of chloramphenicol and acetyl-CoA to chloramphenicol 3-acetate and CoA per min at pH 7.8 at 25 °C.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLBF8205V
106H6812
099F68254
075K8619
074H68351

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Weixin Tao et al.
Journal of microbiology and biotechnology, 21(12), 1203-1210 (2012-01-03)
Function-driven metagenomic analysis is a powerful approach to screening for novel biocatalysts. In this study, we investigated lipolytic enzymes selected from an alluvial soil metagenomic library, and identified two novel esterases, EstDL26 and EstDL136. EstDL26 and EstDL136 reactivated chloramphenicol from
Dian-Li Zhao et al.
Microbial cell factories, 10, 30-30 (2011-05-06)
Pseudoalteromonas is an important genus widespread in marine environment, and a lot of psychrophilic Pseudoalteromonas strains thrive in deep sea and polar sea. By now, there are only a few genetic systems for Pseudoalteromonas reported and no commercial Pseudoalteromonas genetic
Bieke Van Dorst et al.
Journal of applied toxicology : JAT, 32(10), 783-789 (2011-04-19)
Reporter gene assays are commonly used in applied toxicology to measure the transcription of genes involved in toxic responses. In these reporter gene assays, transgenic cells are used, which contain a promoter-operator region of a gene of interest fused to
Zhiyang Li et al.
Journal of nanoscience and nanotechnology, 11(2), 1074-1078 (2011-04-02)
A novel method was established through the detection of chemiluminescent signals of nucleic acid hybridization based on magnetic nanoparticles (MNPs) and PCR. 5' amino- modified specific probes were immobilized on the surface of silanized MNPs by Schiff reaction between amino
Etelvina Figueira et al.
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP, 156(2), 80-86 (2012-05-09)
Since differences in metal accumulation may exist between bivalve species, the aim of this study was to assess the impact of cadmium (Cd) on Ruditapes decussatus and Ruditapes philippinarum. For this, the Cd accumulation, mortality rates and biochemical responses were
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Protocols

Enzymatic Assay of Chloramphenicol Acetyltransferase

To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

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