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Merck
CN

C2900

Chloramphenicol Acetyltransferase from Escherichia coli

lyophilized powder, 50,000-150,000 units/mg protein (Lowry)

Synonym(s):

Acetyl-CoA: chloramphenicol 3-O-acetyltransferase, CAT

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About This Item

CAS Number:
9040-07-7
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
2.3.1.28 (BRENDA, IUBMB)
MDL number:
MFCD00130772

Key Documents

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Product Name

Chloramphenicol Acetyltransferase from Escherichia coli, lyophilized powder, 50,000-150,000 units/mg protein (Lowry)

form

lyophilized powder

specific activity

50,000-150,000 units/mg protein (Lowry)

mol wt

75 kDa (three identical subunits)

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

Gene Information

Escherichia coli ... cat(2847485)

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Biochem/physiol Actions

The enzyme responsible for chloramphenicol resistance in bacteria. Catalyzes the conversion of acetyl-CoA + chloramphenicol to CoA + chloramphenicol 3-acetate.

General description

The enzyme has a molecular weight of approximately 78,000 Da and pH optimum of 7.8.

Other Notes

One unit will convert 1.0 nanomole of chloramphenicol and acetyl-CoA to chloramphenicol 3-acetate and CoA per min at pH 7.8 at 25 °C.

Physical form

Partially purified; contains Tris buffer salts.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLBF8205V
106H6812
099F68254
075K8619
074H68351

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Hirofumi Nariya et al.
Applied and environmental microbiology, 77(23), 8439-8441 (2011-10-04)
A xylose-inducible gene expression vector for Clostridium perfringens was developed. Plasmid pXCH contains a chromosomal region from Clostridium difficile (xylR-P(xy)(lB)): xylR, encoding the xylose repressor, xylO, the xyl operator sequence, and P(xylB), the divergent promoter upstream of xylBA encoding xylulo
Tapan Biswas et al.
Protein science : a publication of the Protein Society, 21(4), 520-530 (2012-02-02)
Novel antibiotics are needed to overcome the challenge of continually evolving bacterial resistance. This has led to a renewed interest in mechanistic studies of once popular antibiotics like chloramphenicol (CAM). Chloramphenicol acetyltransferases (CATs) are enzymes that covalently modify CAM, rendering
Vanessa Fuentes et al.
Eukaryotic cell, 11(6), 725-734 (2012-04-03)
Synthesis of functional mRNA in eukaryotes involves processing of precursor transcripts, including the addition of a poly(A) tail at the 3' end. A multiprotein complex recognizes a polyadenylation signal, generally the hexanucleotide AAUAAA in metazoans, to direct processing of the
Tuija A A Pöyry et al.
Journal of virology, 85(19), 10178-10188 (2011-08-05)
Translation initiation dependent on the foot-and-mouth disease virus (FMDV) internal ribosome entry site (IRES) occurs at two sites (Lab and Lb), 84 nucleotides (nt) apart. In vitro translation of an mRNA comprising the IRES and Lab-Lb intervening segment fused to
Ziyaad Valley-Omar et al.
Virology journal, 8, 462-462 (2011-10-07)
HIV-1 Gag virus like particles (VLPs) used as candidate vaccines are regarded as inert particles as they contain no replicative nucleic acid, although they do encapsidate cellular RNAs. During HIV-1 Gag VLP production in baculovirus-based expression systems, VLPs incorporate the
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Protocols

Enzymatic Assay of Chloramphenicol Acetyltransferase

To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

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