C2900
Chloramphenicol Acetyltransferase from Escherichia coli
lyophilized powder, 50,000-150,000 units/mg protein (Lowry)
Synonym(s):
Acetyl-CoA: chloramphenicol 3-O-acetyltransferase, CAT
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About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
Specific activity:
50,000-150,000 units/mg protein (Lowry)
form
lyophilized powder
Quality Segment
specific activity
50,000-150,000 units/mg protein (Lowry)
mol wt
75 kDa (three identical subunits)
shipped in
dry ice
storage temp.
−20°C
Gene Information
Escherichia coli ... cat(2847485)
General description
The enzyme has a molecular weight of approximately 78,000 Da and pH optimum of 7.8.
Biochem/physiol Actions
The enzyme responsible for chloramphenicol resistance in bacteria. Catalyzes the conversion of acetyl-CoA + chloramphenicol to CoA + chloramphenicol 3-acetate.
Physical form
Partially purified; contains Tris buffer salts.
Other Notes
One unit will convert 1.0 nanomole of chloramphenicol and acetyl-CoA to chloramphenicol 3-acetate and CoA per min at pH 7.8 at 25 °C.
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signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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