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Merck
CN

C2900

Sigma-Aldrich

Chloramphenicol Acetyltransferase from Escherichia coli

lyophilized powder, 50,000-150,000 units/mg protein (Lowry)

Synonym(s):

Acetyl-CoA: chloramphenicol 3-O-acetyltransferase, CAT

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About This Item

CAS Number:
9040-07-7
EC Number:
2.3.1.28 (BRENDA, IUBMB)
MDL number:
MFCD00130772
UNSPSC Code:
12352204
NACRES:
NA.54

Key Documents

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form

lyophilized powder

Quality Level

200

specific activity

50,000-150,000 units/mg protein (Lowry)

mol wt

75 kDa (three identical subunits)

shipped in

dry ice

storage temp.

−20°C

Gene Information

Escherichia coli ... cat(2847485)

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General description

The enzyme has a molecular weight of approximately 78,000 Da and pH optimum of 7.8.

Biochem/physiol Actions

The enzyme responsible for chloramphenicol resistance in bacteria. Catalyzes the conversion of acetyl-CoA + chloramphenicol to CoA + chloramphenicol 3-acetate.

Physical form

Partially purified; contains Tris buffer salts.

Other Notes

One unit will convert 1.0 nanomole of chloramphenicol and acetyl-CoA to chloramphenicol 3-acetate and CoA per min at pH 7.8 at 25 °C.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
SLBF8205V
106H6812
099F68254
075K8619
074H68351

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Protocols

Enzymatic Assay of Chloramphenicol Acetyltransferase

To measure chloramphenicol acetyltransferase activity, this procedure uses DTNB and coenzyme A. The reaction of DTNB with the –SH group on CoA results in a colorimetric increase at 412 nm.

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