C2976
Anti-Caspase 8 (GD-13) antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Sign Into View Organizational & Contract Pricing
Select a Size
About This Item
MDL number:
UNSPSC Code:
12352203
biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen (caspase 8a) 55 kDa
antigen (caspase 8b) 54 kDa
species reactivity
human
technique(s)
microarray: suitable
western blot: 1:1,000 using human Burkitt lymphoma Raji cell extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Gene Information
human ... CASP8(841)
Immunogen
synthetic peptide of the human caspase 8a/MACHα1 precursor (amino acids 362-374 with N-terminal added lysine), conjugated to KLH.
Application
Anti-Caspase 8 (GD-13) is suitable for microarray. It is also suitable for western blot at a concentration of 1:1,000 using human Burkitt lymphoma Raji cell extract.
Biochem/physiol Actions
Caspase 8 cleaves and activates CASP3, CASP4, CASP6, CASP7, CASP9 and CASP10. May participate in the GZMB apoptotic pathways. It also cleaves ADPRT. Caspase 8 hydrolyzes the small-molecule substrate, Ac-Asp-Glu-Val-Asp-|-AMC. It is also a target for cowpox virus CRMA death inhibitory protein. Anti-Caspase 8 (GD-13) recognizes human caspase 8a (55 kDa) and caspase 8b (54kDa) by immunoblotting. Staining of caspase 8 in immunoblotting is specifically inhibited with caspase 8 immunizing peptide (human, amino acids 362-374). Caspases play a crucial role in apoptosis in almost every cell type.
The sequence used as the immunogen corresponds to the C-terminal region of the enzyme p18 subunit and is identical in other human caspase 8 isoforms (MACHα2, MACHα3, MACHβ) and has limited homology (approx. 50%) to mouse caspase 8. By immunoblotting, the antibody reacts with caspase 8a and caspase 8b.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
新产品
This item has
Choose from one of the most recent versions:
Certificates of Analysis (COA)
Lot/Batch Number
Don't see the Right Version?
If you require a particular version, you can look up a specific certificate by the Lot or Batch number.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
D W Nicholson et al.
Trends in biochemical sciences, 22(8), 299-306 (1997-08-01)
Caspases (cysteinyl aspartate-specific proteinases) mediate highly specific proteolytic cleavage events in dying cells, which collectively manifest the apoptotic phenotype. The key and central role that these enzymes play in a biochemical cell-suicide pathway has been conserved throughout the evolution of
Sonja Blasche et al.
PloS one, 8(3), e58937-e58937 (2013-03-22)
Enterohemorrhagic and enteropathogenic E. coli (EHEC and EPEC) can cause severe and potentially life-threatening infections. Their pathogenicity is mediated by at least 40 effector proteins which they inject into their host cells by a type-III secretion system leading to the
Qiang Qiu et al.
Bioactive materials, 21, 483-498 (2022-10-04)
Purinostat Mesylate (PM) is a novel highly selective and active HDAC I/IIb inhibitor, and the injectable formulation of PM (PMF) based on the compound prescription containing cyclodextrin completely can overcome PM's poor solubility and improves its stability and pharmacokinetic properties.
Xuanchuan Wang et al.
American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 18(4), 843-854 (2017-10-05)
Modulation of nitric oxide activity through blockade of CD47 signaling has been shown to reduce ischemia-reperfusion injury (IRI) in various models of tissue ischemia. Here, we evaluate the potential effect of an antibody-mediated CD47 blockade in a syngeneic and an
G M Cohen
The Biochemical journal, 326 ( Pt 1), 1-16 (1997-08-15)
Apoptosis is a major form of cell death, characterized initially by a series of stereotypic morphological changes. In the nematode Caenorhabditis elegans, the gene ced-3 encodes a protein required for developmental cell death. Since the recognition that CED-3 has sequence
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service