recombinant
expressed in E. coli
Assay
≥95% (SDS-PAGE)
form
buffered aqueous solution
specific activity
≥1,500 units/mg protein
mol wt
56 kDa by immunoblotting
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Gene Information
human ... CASP7(840)
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Biochem/physiol Actions
Cleaves the substrates consisting of consensus sequence DEVD.
Member of the CED-3 subfamily, the effector caspases, with an essential role in the apoptotic process. Upon activation, the zymogen is translocated to the mitochondrial and microsomal fractions. It is responsible for the cleavage of cellular substrates that precipitate the morphological changes of apoptosis.
Member of the CED-3 subfamily, the effector caspases; upon activation, the zymogen is translocated to the mitochondrial and microsomal fractions.
Useful in screening caspase inhibitors, studying enzyme regulation, determining specificities of caspase substrates, as well as using as a positive control in caspase activity assays and Western blot analyses.
Physical form
Solution in HEPES, NaCl, DTT and sucrose, without carrier protein
Analysis Note
Migrates as two major polypeptides (20 kDa and 10 kDa) in immunoblotting. Two minor polypeptides derived from the large subunit are also present.
Other Notes
One unit will hydrolyze 1.0 nmol of Ac-Asp-Glu-Val-Asp-AFC per minute at pH 7.5 at 25 °C.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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