C3411
2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein
≥90% (HPLC), Fluorescent indicator, powder
Synonym(s):
BCECF
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About This Item
Empirical Formula (Hill Notation):
C27H20O11
CAS Number:
Molecular Weight:
520.44
NACRES:
NA.32
PubChem Substance ID:
UNSPSC Code:
12352200
MDL number:
Beilstein/REAXYS Number:
8375770
Product Name
2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein, ≥90% (HPLC)
Quality Segment
assay
≥90% (HPLC)
form
powder
storage temp.
−20°C
SMILES string
OC(=O)CCc1cc2c(OC3=CC(=O)C(CCC(O)=O)=CC3=C2c4cc(ccc4C(O)=O)C(O)=O)cc1O
InChI
1S/C27H20O11/c28-19-10-21-17(7-12(19)2-5-23(30)31)25(16-9-14(26(34)35)1-4-15(16)27(36)37)18-8-13(3-6-24(32)33)20(29)11-22(18)38-21/h1,4,7-11,28H,2-3,5-6H2,(H,30,31)(H,32,33)(H,34,35)(H,36,37)
InChI key
DKROGDOAOXDINY-UHFFFAOYSA-N
Application
2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein has been used in a fluorescence-based screening assay, to determine the pH.
Biochem/physiol Actions
2′,7′-Bis(2-carboxyethyl)-5(6)-carboxyfluorescein (BCECF) is useful to study multidrug resistance-associated protein (MRP) transporter action within the cell. BCECF is known to interfere with drug efflux interactions.
Fluorescent indicator for measurement of cytoplasmic pH in lymphocytes.
Storage Class
11 - Combustible Solids
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
PPE (personal protective equipment)
Eyeshields, Gloves, type N95 (US)
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