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Merck
CN

C3545

Monoclonal Anti-Calmodulin antibody produced in mouse

clone 6D4, ascites fluid

Synonym(s):

Anti-CALM3, Anti-calmodulin

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.44
MDL number:
MFCD00162206

Key Documents

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Product Name

Monoclonal Anti-Calmodulin antibody produced in mouse, clone 6D4, ascites fluid

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

6D4, monoclonal

contains

15 mM sodium azide as preservative

species reactivity

chicken, Dictyostelium discoideum, bovine, rat

technique(s)

western blot: 1:2,000 using a bovine brain calmodulin preparation

isotype

IgG1

UniProt accession no.

P0DP29

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

rat ... Calm1(24242)

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Application

Monoclonal Anti-Calmodulin antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblot
  • dot blot
  • immunocytochemistry
  • immunoprecipitation
  • immunofluorescence

Biochem/physiol Actions

Calmodulin is a ubiquitous molecule which can bind up to 4 calcium ions, regulating and mediating a wide variety of biochemical processes.
The antibody recognizes native and SDS-denatured calmodulin.
These antibodies are directed against different epitopes within the calmodulin molecule.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Calmodulin belongs to a family of structurally homologous Ca2+-binding proteins. CALM1 gene is located on human chromosome 14q32.11.
Calmodulin is a highly conserved 17kDa calcium binding protein found in all eukaryotic cells. It is a multifunctional molecule. Monoclonal anti-Calmodulin (mouse IgG1 isotype) is derived from the 6D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with calmodulin, purified from Dictyostelium discoideum and conjugated to KLH.

Immunogen

preparation of purified calmodulin from Dictyostelium discoideum conjugated to KLH.

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Storage Class

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable

Regulatory Information

动植物来源生物产品
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Certificates of Analysis (COA)

Lot/Batch Number
0000193766
0000179090
0000179090
0000193766
019M4880V

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The role of calmodulin and related proteins in plant cell function: an ever-thickening plot
Bergey DR, et al.
Springer Science Reviews, 2(1-2), 145-159 (2014)
Maximal stimulation-induced in situ myosin light chain kinase activity is upregulated in fetal compared with adult ovine carotid arteries
Injeti ER, et al.
American Journal of Physiology. Heart and Circulatory Physiology, 295(6), H2289-H2298 (2008)
The role of calmodulin and related proteins in plant cell function: an ever-thickening plot
Bergey DR, et al.
Springer Science Reviews, 2(1-2), 145-159 (2014)
William D Kim et al.
International journal of molecular sciences, 22(4) (2021-02-13)
Calmodulin (CaM) is an essential calcium-binding protein within eukaryotes. CaM binds to calmodulin-binding proteins (CaMBPs) and influences a variety of cellular and developmental processes. In this study, we used immunoprecipitation coupled with mass spectrometry (LC-MS/MS) to reveal over 500 putative
Association between the polymorphisms of CALM1 gene and osteoarthritis risk: a meta-analysis based on observational studies
Yang H, et al.
Bioscience Reports, 38(5), BSR20181128-BSR20181128 (2018)
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