C3556
Catalase from human erythrocytes
≥90% (SDS-PAGE), buffered aqueous solution, ≥30,000 units/mg protein
Synonym(s):
H2O2:H2O2 oxidoreductase
biological source
human erythrocytes
Quality Level
Assay
≥90% (SDS-PAGE)
form
buffered aqueous solution
specific activity
≥30,000 units/mg protein
mol wt
tetramer ~250 kDa
concentration
≤10 mg/mL
technique(s)
cell based assay: suitable
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
SMILES string
O(CC)C(=O)c1ccc(cc1)O
InChI
1S/C9H10O3/c1-2-12-9(11)7-3-5-8(10)6-4-7/h3-6,10H,2H2,1H3
InChI key
NUVBSKCKDOMJSU-UHFFFAOYSA-N
Gene Information
human ... CAT(847)
General description
Research area: Cell Signaling
Human catalase is a member of the monofunctional heme-containing catalases. It is an intracellular enzyme located at higher concentrations in the liver, erythrocytes, and kidney. Catalase is a homo-tetrameric protein and comprises amino acid residues, one heme group that is iron III protoporphyrin IX, and a nicotinamide adenine dinucleotide phosphate (NADPH) molecule. It is a ubiquitous enzyme found in most aerobic organisms. The catalase (CAT) gene is located on the human chromosome at 11p13.
Human catalase is a member of the monofunctional heme-containing catalases. It is an intracellular enzyme located at higher concentrations in the liver, erythrocytes, and kidney. Catalase is a homo-tetrameric protein and comprises amino acid residues, one heme group that is iron III protoporphyrin IX, and a nicotinamide adenine dinucleotide phosphate (NADPH) molecule. It is a ubiquitous enzyme found in most aerobic organisms. The catalase (CAT) gene is located on the human chromosome at 11p13.
Application
Catalase from human erythrocytes has been used:
- to prevent reoxidation of reduced cytochrome c by H2O2 while measuring the production of superoxide with cytochrome C.
- as a component of the imaging buffer for stochastic optical reconstruction microscopy (STORM) imaging of human skin fibroblasts
- as a component of the gloxy mix for single-molecule imaging
Biochem/physiol Actions
Catalase activates the decomposition of hydrogen peroxide, a reactive oxygen species, into water and oxygen. It functions as a natural antioxidant, protecting cells against oxidative damage to proteins, lipids and nucleic acids. Catalase has also been used to study the role reactive oxygen species play in gene expression and apoptosis.
Deficiency of catalase and oxidative stress are associated with vitiligo, hypertension, Alzheimer′s disease, diabetes mellitus, and acatalasemia. Catalase shows a dichotomous role in cancer as its expression is at a higher level in chronic lymphocytic leukemia, glioma, melanoma, and gastric cancer and lower levels of expression are seen in acute myeloid leukemia, pancreatic, lung, prostate, and skin (non-melanoma) cancers.
Physical form
Solution in 50 mM Tris, pH 8.0
Preparation Note
Tightly closed. Dry. Keep locked up or in an area accessible only to qualified or authorized
persons
persons
Analysis Note
Protein determined by biuret
Other Notes
One unit will decompose 1.0 μmole of H2O2 per min at pH 7.0 at 25 °C, while the H2O2 conc. falls from 10.3 to 9.2 mM, measured by the rate of decrease of A240.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Urte Neniskyte et al.
Methods in molecular biology (Clifton, N.J.), 1041, 103-111 (2013-07-03)
Reactive oxygen and nitrogen species are both regulators and effectors of microglial activation, and assays of these oxidants can be used as a measure of acute and chronic activation of microglial cells. Here we describe quick methods to assess the
Investigation of the binding mechanism and inhibition of bovine liver catalase by quercetin: Multi-spectroscopic and computational study
Samaneh R, et al.
BioImpacts, 7(3), 147?153-147?153 (2017)
D A Chistiakov et al.
Diabetes/metabolism research and reviews, 20(3), 219-224 (2004-05-11)
Oxidative stress is involved in the origin of type 1 diabetes. Low efficiency of the scavenging antioxidant system has been shown to be related to the pathogenesis of the disease. This, therefore suggests that genes encoding catalase and other antioxidant
Li-Wen Shen et al.
Acta pharmacologica Sinica, 43(1), 177-193 (2021-07-24)
Inhibition of autophagy has been accepted as a promising therapeutic strategy in cancer, but its clinical application is hindered by lack of effective and specific autophagy inhibitors. We previously identified cepharanthine (CEP) as a novel autophagy inhibitor, which inhibited autophagy/mitophagy
Marilisa Galasso et al.
Free radical biology & medicine, 172, 264-272 (2021-06-16)
Aerobic organisms possess numerous antioxidant enzymatic families, including catalases, superoxide dismutases (SODs), peroxiredoxins (PRDXs), and glutathione peroxidases (GPXs), which work cooperatively to protect cells from an excess of reactive oxygen species (ROS) derived from endogenous metabolism or external microenvironment. Catalase
Articles
Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
氧化应激，在一部分上，是由多种细胞过程产生的活性氧介导，并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基，如活性氧，会通过细胞引起细胞损伤。
Protocols
This procedure may be used for all Catalase products.
过氧化氢酶催化过氧化氢生成水和氧气。该实验方案使用分光光度法测定过氧化氢酶活性。
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