Control primer set for Arabidopsis Actin-2 gene

This product consists of a primer set for the Arabidopsis housekeeping gene Actin-2 (At3g18780). It is intended for use as an internal control for Q-RT-PCR for expression analysis of any Arabidopsis gene.

Of our three control primer series for Arabidopsis gene expression, Actin-2 control primer is designed as a control for high-abundant gene expression. The other two control primers in the series are nuclear cap-binding protein (CBP20, Product Code C3115) and ubiquitin (UBC, Product Code C3240) designed as controls for medium- to low-abundant gene expression.

Primer should be stored at -20 °C. Primers are stable at -20 °C for at least 1 year and can be frozen and thawed at least 10 times.

Each tube contains enough primer (forward or reverse) for 100 RT-PCR reactions (50 μl reaction volume)

• Forward Primer (T_m = 68 °C based on % GC method in 1 M salt concentration), 20 μM, in TE buffer, Product Code A7728.
• Reverse Primer (T_m = 67 °C based on % GC method in 1 M salt concentration), 20 μM, in TE buffer, Product Code A6978.