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C4018

Sigma-Aldrich

Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

lyophilized powder, ≥100 units/mg protein

Synonym(s):

3α,7α,12α-Trihydroxy-5β-cholan-24-oylglycine amidohydrolase

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1500 UNITS
CN¥2,890.04
3000 UNITS
CN¥6,470.58
7500 UNITS
CN¥10,908.50

CN¥2,890.04

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1500 UNITS
CN¥2,890.04
3000 UNITS
CN¥6,470.58
7500 UNITS
CN¥10,908.50

About This Item

CAS Number:
37289-07-9
EC Number:
3.5.1.24 (BRENDA, IUBMB)
MDL number:
MFCD00130789
UNSPSC Code:
12352204
NACRES:
NA.54

CN¥2,890.04

In StockDetails

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form

lyophilized powder

specific activity

≥100 units/mg protein

composition

Protein, ≥50% biuret

shipped in

wet ice

storage temp.

−20°C

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This Item
G2878C1129T9034
Choloylglycine Hydrolase from Clostridium perfringens (C. welchii) lyophilized powder, ≥100 units/mg protein

C4018

Choloylglycine Hydrolase from Clostridium perfringens (C. welchii)

Glycocholic acid hydrate synthetic, ≥97% (HPLC)

G2878

Glycocholic acid hydrate

Cholic acid from bovine and/or ovine, ≥98%

C1129

Cholic acid

Taurocholic acid sodium salt hydrate BioXtra, ≥95% (HPLC)

T9034

Taurocholic acid sodium salt hydrate

specific activity

≥100 units/mg protein

specific activity

-

specific activity

-

specific activity

-

form

lyophilized powder

form

powder

form

powder

form

powder

storage temp.

−20°C

storage temp.

room temp

storage temp.

room temp

storage temp.

room temp

shipped in

wet ice

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

composition

Protein, ≥50% biuret

composition

-

composition

-

composition

-

Application

The enzyme from Sigma has been used in the analysis of bile samples in various studies.[1][2][3]

Biochem/physiol Actions

The enzyme is an N-terminal nucleophilic (Ntn) hydrolase that catalyzes the hydrolysis of amide bonds, liberates the glycine/taurine moiety from the steroid core and eventually yields unconjugated bile acids. Agents that oxidize thiol groups (e.g., p-mercuribenzoate, iodoacetamide, Hg2+, Cu2+, and Cd2+) have been shown to strongly inhibit bile salt hydrolase (BSH) activity in Clostridium perfringens.[4]

Unit Definition

One unit will hydrolyze 1.0 μmole of glycocholic acid to glycine and cholic acid in 5 min at pH 5.6 at 37 °C.

Physical form

Partially purified lyophilized powder containing buffer salts and stabilizer

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000282850
    0000142044
    0000188551
    0000282850
    0000188551

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    Effects of Quercetin and Rutin on Serum and Hepatic Lipid Concentrations, Fecal Steroid Excretion and Serum Antioxidant Properties
    Nakamura Y, et al.
    Journal of Health Science, 46(4), 229-240 (2000)
    Bile salt hydrolase activity in probiotics.
    Máire Begley et al.
    Applied and environmental microbiology, 72(3), 1729-1738 (2006-03-07)
    Maksim Rossocha et al.
    Biochemistry, 44(15), 5739-5748 (2005-04-13)
    Bacterial bile salt hydrolases catalyze the degradation of conjugated bile acids in the mammalian gut. The crystal structures of conjugated bile acid hydrolase (CBAH) from Clostridium perfringens as apoenzyme and in complex with taurodeoxycholate that was hydrolyzed to the reaction
    Konstantinos C Mountzouris et al.
    Clinical nutrition (Edinburgh, Scotland), 28(3), 318-324 (2009-02-19)
    Little is known about fasting effects on gut bacterial metabolism. As probiotics are purported to be beneficial for health, this study aimed to investigate the response of gut microbial metabolism on fasting with or without probiotic administration. Sixty male adult
    F Kannenberg et al.
    The Journal of biological chemistry, 274(50), 35455-35460 (1999-12-10)
    Peroxisomal beta-oxidation plays an important role in the metabolism of a wide range of substrates, including various fatty acids and the steroid side chain in bile acid synthesis. Two distinct thiolases have been implicated to function in peroxisomal beta-oxidation: the
    View All Related Papers

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