C4356
Anti-Caspase 9 antibody,Mouse monoclonal
clone CAS9, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
CAS9, monoclonal
form
buffered aqueous solution
mol wt
antigen 37 kDa
species reactivity
human
concentration
~2 mg/mL
technique(s)
indirect ELISA: suitable
microarray: suitable
western blot: 0.5-1 μg/mL using a purified recombinant human caspase 9 preparation
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CASP9(842)
General description
Monoclonal Anti-Caspase 9 (mouse IgG1 isotype) is derived from the CAS9 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a synthetic peptide corresponding to amino acids of human caspase 9 protein, conjugated to keyhole limpet hemocyanin (KLH). Caspases (cysteine-requiring aspartate protease) are a family of proteases that share similarities in amino acid sequences, structure and substrate specificity. Caspases are normally present in the cell as inactive procaspases. Caspase 9 (also known as Mch6 and ICE-LAP6) gene encodes a protein product of 37 kDa (reported also as 46 kDa), which undergoes a cleavage upon activation, leading to p18/p15/p9 subunits. It is a member of the CED-3 subfamily, bearing high similarity to caspase. High level expression of caspase 9 is found in the heart, testis and ovary.
Immunogen
synthetic peptide corresponding to amino acids 299-316 of human caspase 9 protein conjugated to KLH.
Application
Anti-Caspase 9 antibody,Mouse monoclonal has been used in:
- western blot assay
- immunocytochemistry
- enzyme-linked immunosorbent assay (ELISA).
Biochem/physiol Actions
Caspase 9 is a downstream protease, activated in caspase 3- and granzyme B-mediated apoptosis. Procaspase 9 is activated when a complex is formed with the cofactor apoptotic protease activating factor-1 (Apaf-1) through the caspase recruitment domain (CARD). Caspase 9 plays a critical role in the survival of neuronal progenitors within the embryonic forebrain and cortex. Its deficiency leads to defective brain development and embryonic lethality. Overexpression of caspase 9, but not of a mutant in which the catalytic Cys was replaced with an Ala, induces apoptosis in MCF7 cells.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Information
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Generation of Advanced Glycation End-Products (AGEs) by glycoxidation mediated by copper and ROS in a human serum albumin (HSA) model peptide: reaction mechanism and damage in motor neuron cells
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Mutation Research. Genetic Toxicology and Environmental Mutagenesis, 824(6), 42-51 (2017)
Neural-specific inactivation of ShcA results in increased embryonic neural progenitor apoptosis and microencephaly
McFarland KN, et al.
The Journal of Neuroscience, 26(30), 7885-7897 (2006)
Apoptosis dependent and independent functions of caspases
Madame Curie Bioscience Database [Internet]. (2013)
CD 47 blockade reduces ischemia/reperfusion injury in donation after cardiac death rat kidney transplantation
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American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 18(4), 843-854 (2018)
Role of caspases in the regulation of apoptotic pancreatic islet beta-cells death
Hui H, et al.
Journal of Cellular Physiology, 200(2), 177-200 (2004)
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