C4856
Anti-CASK/LIN2 (KA-17) antibody produced in rabbit
affinity isolated antibody, buffered aqueous solution
biological source
rabbit
conjugate
unconjugated
antibody form
affinity isolated antibody
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen ~112 kDa
species reactivity
canine, human
technique(s)
immunoprecipitation (IP): 5-10 μg using lysate of 3x105 cultured MDCK cells
microarray: suitable
western blot (chemiluminescent): 1:300 using whole cell extract of cultured human Jurkat and dog MDCK kidney cells
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... CASK(8573)
General description
CASK/Lin-2 (calcium/calmodulin-dependent serine kinase) has an N-terminal domain similar to calcium-calmodulin dependent protein kinase (CAM), a Lin-7 binding domain, a single PDZ domain, an SH3 domain, and a C-terminal GUK (guanylate kinase) homologous domain. CASK/Lin-2 gene is mapped to human chromosome Xp11.4.
Calcium/Calmodulin-dependent Serine Kinase (CASK/Lin-2) is predominant in the brain, may be ubiquitously expressed. It localizes to the lateral and/or basal plasma membrane regions in epithelial cells.
Immunogen
synthetic peptide corresponding to human CASK/LIN-2 sequence, (amino acids 393-409) conjugated to KLH.
Application
Anti-CASK/LIN2 (KA-17) antibody produced in rabbit is suitable for:
- immunoprecipitation at a concentraqtion of 5-10μg, using lysate of 3x105 cultured MDCK cells
- western blot (chemiluminescent) at a dilution of 1:300, using whole cell extract of cultured human Jurkat and dog MDCK kidney cells
Biochem/physiol Actions
Anti-CASK/Lin-2 specifically recognizes humanCASK/Lin-2.
CASK/Lin-2 (Calcium/Calmodulin-dependent Serine Kinase) is translocated into the nucleus to regulate gene expression. It interacts via its PDZ domain with the cytosolic tail of the transmembrane cell surface heparin sulfate proteoglycan Syndecan-2. In neurons it interacts with neurexins. In addition, CASK/Lin-2 is capable of interacting with the actinbinding band 1 protein, neuronal munc18-1-interacting protein 1(Mint-1/Lin-10), protein lin-7 homolog A (Veli/Lin-7) proteins, junctional adhesion molecule (JAM), disks large homolog 1 (hDlg), and the nuclear T-box brain transcription factor 1 (Tbr-1).
Mutations in the CASK gene is implicated in the X-linked brain malformation syndrome. A missense mutation in the CASK gene is also reported in the pathophysiology of FG syndrome.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide.
Preparation Note
For continuous use, store at 2-8 °C for up to one month.For prolonged storage, freeze in working aliquots at−20 °C. Repeated freezing and thawing is not recom-mended. Storage in frost-free freezers is also notrecommended. If slight turbidity occurs upon prolongedstorage, clarify the solution by centrifugation beforeuse. Working dilutions should be discarded if not usedwithin 12 hours.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Sorting out genes that regulate epithelial and neuronal polarity.
D S Bredt
Cell, 94(6), 691-694 (1998-09-30)
Y P Hsueh et al.
Nature, 404(6775), 298-302 (2000-04-05)
Membrane-associated guanylate kinases (MAGUKs) contain multiple protein-binding domains that allow them to assemble specific multiprotein complexes in particular regions of the cell. CASK/LIN-2, a MAGUK required for EGF receptor localization and signalling in Caenorhabditis elegans, contains a calmodulin-dependent protein kinase-like
The location of human CASK at Xp11.4 identifies this gene as a candidate for X-linked optic atrophy.
S D Dimitratos et al.
Genomics, 51(2), 308-309 (1998-09-02)
J P Borg et al.
The Journal of biological chemistry, 273(48), 31633-31636 (1998-11-21)
In Caenorhabditis elegans, lin-2, lin-7, and lin-10 genetically interact to control the trafficking of the Let-23 growth factor receptor to the basolateral surface of body epithelia. The human homologue of the lin-10 gene has recently been identified as a member
Y Hata et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 16(8), 2488-2494 (1996-04-15)
Neurexins are neuronal cell surface proteins with hundreds of isoforms. In yeast two-hybrid screens for intracellular molecules interacting with different neurexins, we identified a single interacting protein called CASK. CASK is composed of an N-terminal Ca2+, calmodulin-dependent protein kinase sequence
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