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Merck
CN

C5921

Sigma-Aldrich

Cholesterol Esterase from bovine pancreas

lyophilized powder, ≥10,000 units/g protein

Synonym(s):

Sterol-ester acylhydrolase

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About This Item

CAS Number:
EC Number:
EC Number:
MDL number:
UNSPSC Code:
12352204
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form

lyophilized powder

specific activity

≥10,000 units/g protein

storage temp.

−20°C

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Application

Enzyme responsible for the hydrolysis of many of the fatty acid esters of cholesterol.
Optimum pH range: 6-8
Activators: Bisphenol A diglycidyl ether, cAMP-dependent protein kinase type II, ethanol, methanol, n-butanol, phosphatidylcholine, phosphatidylethanolamine, sodium taurocholic acid
Inhibitors: Bisphenol A methacrylate, diisopropylfluorophosphate, enolase, Hg2+, sodium fluoride, phosphatidic acid, phosphatidylcholine, phosphatidylserine

Analysis Note

Protein determined by biuret.

Other Notes

One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per min at pH 7.0 at 37 °C in the presence of taurocholate.

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Precautionary Statements

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Information

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Maria J Pereira et al.
Molecular and cellular endocrinology, 365(2), 260-269 (2012-11-20)
Cyclosporin A (CsA), tacrolimus and rapamycin are immunosuppressive agents (IAs) associated with insulin resistance and dyslipidemia, although their molecular effects on lipid metabolism in adipose tissue are unknown. We explored IAs effects on lipolysis, lipid storage and expression of genes
Myung Hee Moon et al.
Experimental & molecular medicine, 44(10), 603-614 (2012-08-04)
Higher levels of body fat are associated with an increased risk for development numerous adverse health conditions. FTY720 is an immune modulator and a synthetic analogue of sphingosine 1-phosphate (S1P), activated S1P receptors and is effective in experimental models of
Joanna Karbowska et al.
Steroids, 77(13), 1359-1365 (2012-09-07)
Dehydroepiandrosterone (DHEA) reduces body fat in rodents and humans, and increases glycerol release from isolated rat epididymal adipocytes and human visceral adipose tissue explants. It suggests that DHEA stimulates triglyceride hydrolysis in adipose tissue; however, the mechanisms underlying this action
Yong-lan Huang et al.
Zhonghua er ke za zhi = Chinese journal of pediatrics, 50(8), 601-605 (2012-11-20)
To explore the clinical characteristics of Wolman disease and diagnostic methods using enzymatic and molecular analysis. Lysosomal acid lipase activity was measured using 4-methylumbelliferyl oleate in the leukocytes of an infant suspected of Wolman disease and LIPA gene mutational analysis
Vincent Delorme et al.
PloS one, 7(9), e46493-e46493 (2012-10-03)
Lipid metabolism plays an important role during the lifetime of Mycobacterium tuberculosis, the causative agent of tuberculosis. Although M. tuberculosis possesses numerous lipolytic enzymes, very few have been characterized yet at a biochemical/pharmacological level. This study was devoted to the

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