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Merck
CN

C6111

Sigma-Aldrich

Anti-m-Calpain (Domain III) antibody produced in rabbit

~1 mg/mL, affinity isolated antibody, buffered aqueous glycerol solution

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About This Item

MDL number:
MFCD03455418
UNSPSC Code:
12352203

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biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

clone

polyclonal

form

buffered aqueous glycerol solution

species reactivity

human, mouse, rat

concentration

~1 mg/mL

technique(s)

immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot (chemiluminescent): 1:5,000
western blot: 1:1,000

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... CAPN3(825)
mouse ... Capn3(12335)
rat ... Capn3(29155)

Immunogen

synthetic peptide corresponding to the C-terminal end of domain-III of the large subunit of human m-calpain (calpain-II, protein kinase C activating factor, E.C. 3.4.22.17).

Biochem/physiol Actions

Recognizes latent and active m-calpain. Binds the reduced and non-reduced protein. Identifies bands at 80 kDa, 58 kDa, and a series of further cleaved active forms.

Physical form

Solution in 0.01 M phosphate buffered saline, containing 50% glycerol and 0.05% sodium azide.

Regulatory Information

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Certificates of Analysis (COA)

Lot/Batch Number
055K1042
114K1239
112K1472
012K1375

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