C7149
Cholesterol Oxidase from Pseudomonas fluorescens
lyophilized powder, 10-50 units/mg protein
Synonym(s):
Cholesterol: oxygen oxidoreductase
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About This Item
CAS Number:
UNSPSC Code:
12352204
EC Number:
232-842-1
MDL number:
form
lyophilized powder
specific activity
10-50 units/mg protein
composition
Protein, ~15% biuret
storage temp.
−20°C
Other Notes
Contains sodium cholate and borate.
One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization.
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Ruimin Li et al.
Analytica chimica acta, 724, 80-85 (2012-04-10)
A novel colorimetric method for detection of cholesterol was developed with hemin-G-quadruplex DNAzyme by transducing oxidation of cholesterol into the color change of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS(2-)). Oligonucleotide 5'-GTGGGTAGGGCGGGTTGG-3' (Oligo-1) formed G-quadruplex structure in the presence of K(+), it
Marcos Eguílaz et al.
Analytical chemistry, 83(20), 7807-7814 (2011-09-13)
The design of a novel biosensing electrode surface, combining the advantages of magnetic ferrite nanoparticles (MNPs) functionalized with glutaraldehyde (GA) and poly(diallyldimethylammonium chloride) (PDDA)-coated multiwalled carbon nanotubes (MWCNTs) as platforms for the construction of high-performance multienzyme biosensors, is reported in
J MacLachlan et al.
The Journal of steroid biochemistry and molecular biology, 72(5), 169-195 (2000-05-24)
Since Flegg (H.M. Flegg, An investigation of the determination of serum cholesterol by an enzymatic method, Ann. Clin. Biochem. 10 (1973) 79-84) and Richmond (W. Richmond, The development of an enzymatic technique for the assay of cholesterol in biological fluids
Anna Brzostek et al.
The Journal of steroid biochemistry and molecular biology, 134, 1-7 (2012-10-16)
Cholesterol degradation is achieved through a complex metabolic pathway that starts with the oxidation of the 17-alkyl side chain and the steroid ring system. In bacteria, the oxidation of the 3β-hydroxyl group and isomerization of the resulting cholest-5-en-3-one to cholest-4-en-3-one
Tatyana V Ivashina et al.
The Journal of steroid biochemistry and molecular biology, 129(1-2), 47-53 (2011-10-22)
Fast-growing strain of Mycobacterium sp. VKM Ac-1815D is capable of effective oxidizing of sterols (phytosterol, cholesterol, ergosterol) to androstenedione and other valuable 3-oxo-steroids. To elucidate the role of cholesterol oxidase in sterol catabolism by the strain, the choD gene has
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