C7149
Cholesterol Oxidase from Pseudomonas fluorescens
lyophilized powder, 10-50 units/mg protein
Synonym(s):
Cholesterol: oxygen oxidoreductase
form
lyophilized powder
specific activity
10-50 units/mg protein
composition
Protein, ~15% biuret
storage temp.
−20°C
Other Notes
Contains sodium cholate and borate.
One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Information
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Preparation and properties of a cholesterol oxidase from Nocardia sp. and its application to the enzymatic assay of total cholesterol in serum.
W Richmond
Clinical chemistry, 19(12), 1350-1356 (1973-12-01)
Silvan Türkcan et al.
Biophysical journal, 102(10), 2299-2308 (2012-06-09)
We track single toxin receptors on the apical cell membrane of MDCK cells with Eu-doped oxide nanoparticles coupled to two toxins of the pore-forming toxin family: α-toxin of Clostridium septicum and ε-toxin of Clostridium perfringens. These nonblinking and photostable labels
Chengyan Wang et al.
Talanta, 94, 263-270 (2012-05-23)
A novel scheme for the fabrication of gold/platinum hybrid functionalized ZnO nanorods (Pt-Au@ZnONRs) and multiwalled carbon nanotubes (MWCNTs) modified electrode is presented and its application for cholesterol biosensor is investigated. Firstly, Pt-Au@ZnONRs was prepared by the method of chemical synthesis.
Sowmya Viswanathan et al.
Methods in enzymology, 509, 165-194 (2012-05-10)
Design of a unique, single-platform, integrated, multichannel sensor based on carbon nanotube (CNT)-protein adducts specific to each one of the major analytes of blood, glucose, cholesterol, triglyceride, and Hb1AC is presented. The concept underlying the sensor, amperometric detection, is applicable
Leah K Cuddy et al.
Journal of neurochemistry, 128(5), 725-740 (2013-10-17)
The sodium-coupled, hemicholinium-3-sensitive, high-affinity choline transporter (CHT) is responsible for transport of choline into cholinergic nerve terminals from the synaptic cleft following acetylcholine release and hydrolysis. In this study, we address regulation of CHT function by plasma membrane cholesterol. We
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