Skip to Content
Merck
CN

C7149

Cholesterol Oxidase from Pseudomonas fluorescens

lyophilized powder, 10-50 units/mg protein

Synonym(s):

Cholesterol: oxygen oxidoreductase

Sign In to View Organizational & Contract Pricing.

Select a Size


About This Item

CAS Number:
UNSPSC Code:
12352204
EC Number:
232-842-1
MDL number:
EC Number:
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

form

lyophilized powder

specific activity

10-50 units/mg protein

composition

Protein, ~15% biuret

storage temp.

−20°C

Other Notes

Contains sodium cholate and borate.
One unit will convert 1.0 μmole of cholesterol to 4-cholesten-3-one per min at pH 7.5 at 25 °C. Note: 4-cholesten-3-one may undergo isomerization.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Regulatory Information

新产品
This item has

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Ruimin Li et al.
Analytica chimica acta, 724, 80-85 (2012-04-10)
A novel colorimetric method for detection of cholesterol was developed with hemin-G-quadruplex DNAzyme by transducing oxidation of cholesterol into the color change of 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS(2-)). Oligonucleotide 5'-GTGGGTAGGGCGGGTTGG-3' (Oligo-1) formed G-quadruplex structure in the presence of K(+), it
Marcos Eguílaz et al.
Analytical chemistry, 83(20), 7807-7814 (2011-09-13)
The design of a novel biosensing electrode surface, combining the advantages of magnetic ferrite nanoparticles (MNPs) functionalized with glutaraldehyde (GA) and poly(diallyldimethylammonium chloride) (PDDA)-coated multiwalled carbon nanotubes (MWCNTs) as platforms for the construction of high-performance multienzyme biosensors, is reported in
J MacLachlan et al.
The Journal of steroid biochemistry and molecular biology, 72(5), 169-195 (2000-05-24)
Since Flegg (H.M. Flegg, An investigation of the determination of serum cholesterol by an enzymatic method, Ann. Clin. Biochem. 10 (1973) 79-84) and Richmond (W. Richmond, The development of an enzymatic technique for the assay of cholesterol in biological fluids
Anna Brzostek et al.
The Journal of steroid biochemistry and molecular biology, 134, 1-7 (2012-10-16)
Cholesterol degradation is achieved through a complex metabolic pathway that starts with the oxidation of the 17-alkyl side chain and the steroid ring system. In bacteria, the oxidation of the 3β-hydroxyl group and isomerization of the resulting cholest-5-en-3-one to cholest-4-en-3-one
Tatyana V Ivashina et al.
The Journal of steroid biochemistry and molecular biology, 129(1-2), 47-53 (2011-10-22)
Fast-growing strain of Mycobacterium sp. VKM Ac-1815D is capable of effective oxidizing of sterols (phytosterol, cholesterol, ergosterol) to androstenedione and other valuable 3-oxo-steroids. To elucidate the role of cholesterol oxidase in sterol catabolism by the strain, the choD gene has

Related Content

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service