C7275
Concanavalin A from Canavalia ensiformis (Jack bean)
Type V, lyophilized powder
Synonym(s):
Con A
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
About This Item
type
Type V
form
lyophilized powder
potency
≤64 μg per mL agglutination activity
impurities
Carbohydrate, essentially free, salt, essentially free, ≤0.16 wt. % Ca, ≤0.23 wt. % Mn
storage temp.
−20°C
Biochem/physiol Actions
Con A is not blood group specific but has an affinity for terminal α-D-mannosyl and α-D-glucosyl residues. Ca2+ and Mn2+ ions are required for activity. Con A dissociates into dimers at pH 5.6 or below. Between pH 5.8 and pH 7.0, Con A exists as a tetramer; above pH 7.0 higher aggregates are formed. Con A exhibits mitogenic activity which is dependent on its degree of aggregation. Succinylation results in an active dimeric form which remains a dimer above pH 5.6.
Preparation Note
Produced from whole Jack beans (J0250) using the method of Sophianopoulos.
Analysis Note
Highly purified.
Where reported, agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, containing Ca2+ and Mn2+ of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hr incubation at 25 °C.
Still not finding the right product?
Explore all of our products under Concanavalin A from Canavalia ensiformis (Jack bean)
signalword
Danger
hcodes
Hazard Classifications
Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Regulatory Information
新产品
This item has
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Related Content
Product Information Sheet
A J Sophianopoulos et al.
Preparative biochemistry, 11(4), 413-435 (1981-01-01)
Concanavalin A (Con A), obtained either commercially or by affinity chromatography, was further purified by incubating at 6-8 hr at pH 3.0-3.2 in 1 M NaCl, 0.08 M glycine and 3 mM each Ca2+ and Mn+2, heat treating at 45
Guanbin Gao et al.
Traffic (Copenhagen, Denmark), 21(3), 274-296 (2019-12-29)
Protein retention and the transport of proteins and lipids into and out of the Golgi is intimately linked to the biogenesis and homeostasis of this sorting hub of eukaryotic cells. Of particular importance are membrane proteins that mediate membrane fusion
Gustavo Vasen et al.
Communicative & integrative biology, 13(1), 128-139 (2020-10-06)
Chemotactic/chemotropic cells follow accurately the direction of gradients of regulatory molecules. Many G-protein-coupled receptors (GPCR) function as chemoattractant receptors to guide polarized responses. In "a" mating type yeast, the GPCR Ste2 senses the α-cell's pheromone. Previously, phosphorylation and trafficking of