C7901
Chelex® 100 sodium form
50-100 mesh (dry)
Synonym(s):
Iminodiacetic Acid Resin
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About This Item
form
beads
Quality Level
matrix
1% cross-linked polystyrene, cross-linked polystyrene beads
matrix active group
iminodiacetic acid (sodium form), paired iminodiacetate groups
matrix attachment
through methyl group to aromatic rings
particle size
50-100 mesh (dry)
pH
4-14
capacity
≥0.3 meq/mL binding capacity, 0.6 meq/g binding capacity (heavy metal ions)
Application
Chelex® 100 sodium form has been used:
- for the extraction of DNA from bee samples
- to remove from cigarette smoke extract (CSE) and study the effects of hepcidin expression in the murine lungs
- to extract DNA from bacterial cultures
Chelex is used in affinity chromatography, protein chromatography, chelating resins, affinity resins and core bioreagents.
Biochem/physiol Actions
Chelex® 100 is a styrene-divinylbenzene copolymer containing paired iminodiacetate ions, which act as chelating groups in binding polyvalent metal ions. It is considered a weakly acidic resin. Chelex acts as a chelating resin that is used in DNA extraction from forensic samples, such as hair, bloodstain cards, and buccal swabs.
Other Notes
Product is autoclavable in sodium form. It has a maximum operating temperature of 75°C and is not soluble in water.
Legal Information
Chelex is a registered trademark of Bio-Rad Laboratories, Inc.
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Storage Class
11 - Combustible Solids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
Product Information Sheet
T P Ryan et al.
Journal of biochemical toxicology, 8(1), 33-39 (1993-03-01)
The effects of transition metals on nonenzymatic and ceruloplasmin catalyzed epinephrine oxidation were investigated by studying rates of epinephrine oxidation in purified buffers and in the presence of metal chelating agents. We found that epinephrine does not "autoxidize" in sodium
Methods for extracting genomic DNA from whole blood samples: current perspectives
Chacon Cortes D, et al.
Journal of Biosciences, 2014(2), 1-9 (2014)
Elizabeth Perez et al.
Journal of immunology (Baltimore, Md. : 1950), 205(9), 2489-2498 (2020-09-23)
Chronic obstructive pulmonary disease (COPD) is a debilitating lung disease associated with cigarette smoking. Alterations in local lung and systemic iron regulation are associated with disease progression and pathogenesis. Hepcidin, an iron regulatory peptide hormone, is altered in subjects with
Global Trade Item Number
| SKU | GTIN |
|---|---|
| C7901-25G | 04061835515899 |
| C7901-100G | 04061835572328 |
| C7901-50G | 04061835572342 |
| C7901-500G | 04061835572335 |